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Sporotrichosis

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181. Subcutaneous mycoses in coastal Karnataka in south India. (PubMed)

Karnataka, India.Between January 2005 and January 2013, a total of 25 patients were diagnosed with subcutaneous mycoses based on a detailed clinical history and presentation, histopathology, and culture of organisms.Chromoblastomycosis was the infection most commonly seen (n = 16 patients, 64%), followed by mycetoma (n = 4, 16%), sporotrichosis (n = 4, 16%), and rhinoentomophthoromycosis (n = 1, 4%). The extremities were the most common site of involvement, with the lower limb being most affected (64

2015 International Journal of Dermatology

182. Respiratory Failure due to Possible Donor-Derived Sporothrix schenckii Infection in a Lung Transplant Recipient (PubMed)

after transplant in a bilateral single lung transplant recipient. This is the first case report of sporotrichosis in a lung transplant recipient. Our patient responded well to amphotericin induction therapy followed by maintenance therapy with itraconazole. The implications of donor-derived fungal infections and Sporothrix in transplant recipients are reviewed. Early recognition and management of these fungi are essential in improving outcomes.

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2015 Case reports in infectious diseases

183. Molecular Diagnosis of Pathogenic Sporothrix Species (PubMed)

Molecular Diagnosis of Pathogenic Sporothrix Species Sporotrichosis is a chronic (sub)cutaneous infection caused by thermodimorphic fungi in the order, Ophiostomatales. These fungi are characterized by major differences in routes of transmission, host predilections, species virulence, and susceptibilities to antifungals. Sporothrix species emerge in the form of outbreaks. Large zoonoses and sapronoses are ongoing in Brazil and China, respectively. Current diagnostic methods based on morphology (...) . brasiliensis, S. schenckii, S. globosa, S. mexicana, S. pallida, and O. stenoceras. We employed a murine model of disseminated sporotrichosis to optimize a PCR assay for detecting Sporothrix in clinical specimens.Primer-BLAST searches revealed candidate sequences that were conserved within a single species. Species-specific primers showed no significant homology with human, mouse, or microorganisms outside the Sporothrix genus. The detection limit was 10-100 fg of DNA in a single round of PCR

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2015 PLoS neglected tropical diseases

184. Construction and analysis of the cDNA subtraction library of yeast and mycelial phases of Sporothrix globosa isolated in China: identification of differentially expressed genes (PubMed)

Construction and analysis of the cDNA subtraction library of yeast and mycelial phases of Sporothrix globosa isolated in China: identification of differentially expressed genes Species included in the Sporothrix schenckii complex are temperature-dependent with dimorphic growth and cause sporotrichosis that is characterized by chronic and fatal lymphocutaneous lesions. The putative species included in the Sporothrix complex are S. brasiliensis, S. globosa, S. mexicana, S. pallida, S. schenckii (...) , and S. lurei. S. globosa is the causal agent of sporotrichosis in China, and its pathogenicity appears to be closely related to the dimorphic transition, i.e. from the mycelial to the yeast phase, it adapts to changing environmental conditions. To determine the molecular mechanisms of the switching process that mediates the dimorphic transition of S. globosa, suppression subtractive hybridization (SSH) was used to prepare a complementary DNA (cDNA) subtraction library from the yeast and mycelial

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2015 Journal of Zhejiang University. Science. B

185. Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification (PubMed)

Rapid Identification of Emerging Human-Pathogenic Sporothrix Species with Rolling Circle Amplification Sporothrix infections are emerging as an important human and animal threat among otherwise healthy patients, especially in Brazil and China. Correct identification of sporotrichosis agents is beneficial for epidemiological surveillance, enabling implementation of adequate public-health policies and guiding antifungal therapy. In areas of limited resources where sporotrichosis is endemic, high

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2015 Frontiers in microbiology

186. Phenotypic Characteristics Associated with Virulence of Clinical Isolates from the Sporothrix Complex (PubMed)

Phenotypic Characteristics Associated with Virulence of Clinical Isolates from the Sporothrix Complex The Sporothrix complex members cause sporotrichosis, a subcutaneous mycosis with a wide spectrum of clinical manifestations. Several specific phenotypic characteristics are associated with virulence in many fungi, but studies in this field involving the Sporothrix complex species are scarce. Melanization, thermotolerance, and production of proteases, catalase, and urease were investigated in 61 (...) S. brasiliensis, one S. globosa, and 10 S. schenckii strains. The S. brasiliensis strains showed a higher expression of melanin and urease compared with S. schenckii. These two species, however, presented similar thermotolerances. Our S. globosa strain had low expression of all studied virulence factors. The relationship between these phenotypes and clinical aspects of sporotrichosis was also evaluated. Strains isolated from patients with spontaneous regression of infection were heavily

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2015 BioMed research international

187. The difficult management of disseminated Sporothrix brasiliensis in a patient with advanced AIDS (PubMed)

The difficult management of disseminated Sporothrix brasiliensis in a patient with advanced AIDS Sporotrichosis is an infection caused by a dimorphic fungus of the Sporothrix schenckii complex. Host immunity is an important factor in the clinical manifestations of the disease. Deeply immunocompromised individuals, especially those infected with the Human Immunodeficiency Virus (HIV) and T CD4 counts < 350 cells/ul lymphocytes, may present with the systemic form of sporotrichosis. This report (...) describes a case of disseminated sporotrichosis caused by S. brasiliensis in a patient with advanced AIDS. The skin, lungs, bones and central nervous system were affected. Medical treatment involved the administration of amphotericin B, terbinafine, itraconazole and posaconazole. Posaconazole was associated with the best clinical response and clearing of the fungus from the central nervous system.

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2015 AIDS research and therapy

188. Miltefosine is active against Sporothrix brasiliensis isolates with in vitro low susceptibility to amphotericin B or itraconazole. (PubMed)

Miltefosine is active against Sporothrix brasiliensis isolates with in vitro low susceptibility to amphotericin B or itraconazole. Sporotrichosis is a common mycosis caused by dimorphic fungi from the Sporothrix schenckii complex. In recent years, sporotrichosis incidence rates have increased in the Brazilian state of Rio de Janeiro, where Sporothrix brasiliensis is the species more frequently isolated from patients. The standard antifungals itraconazole and amphotericin B are recommended (...) as first-line therapy for cutaneous/lymphocutaneous and disseminated sporotrichosis, respectively, although decreased sensitivity to these drugs in vitro was reported for clinical isolates of S. brasiliensis. Here, we evaluated the activity of the phospholipid analogue miltefosine - already in clinical use against leishmaniasis - towards the pathogenic yeast form of S. brasiliensis isolates with low sensitivity to itraconazole or amphotericin B in vitro. Miltefosine had fungicidal activity

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2015 Journal of Medical Microbiology

189. Skin and subcutaneous infections in south-east Asia. (PubMed)

Skin and subcutaneous infections in south-east Asia. We reviewed current literature on four different skin and subcutaneous infections which are often touted as 'emerging diseases' of south-east Asia, namely melioidosis, penicilliosis, sporotrichosis and Mycobacterium marinum infection. Lack of consensus treatment guidelines, high treatment costs and limited investigative capability in certain endemic areas are among the challenges faced by managing physicians. With the increase in borderless (...) travelling, it is hoped that this review will facilitate better understanding and heighten the clinical suspicion of such infections for clinicians in other parts of the world.An increasing number of cases of melioidosis and penicilliosis have been reported within and outside its endemic areas, but epidemiological data on sporotrichosis and M. marinum infections are still sparse. The clinical features of these infections remain historically unchanged and more disseminated infections are seen particularly

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2015 Current Opinion in Infectious Diseases

190. Higher sensitivity of immunohistochemistry for bona fide diagnosis of dog Leishmania (Viannia) braziliensis - driven American tegumentary leishmaniasis: description of an optimized immunohistochemistry method. (PubMed)

of IHC for the detection of amastigote forms and other Leishmania sp. antigen-positive cells and compares the results of IHC, histopathology and cytopathology for the diagnosis of canine ATL. In addition, possible cross-reactivity with sporotrichosis is analyzed.Forty paraffin-embedded biopsies and 40 smears of cutaneous lesions from dogs with ATL, confirmed by isolation and characterization of Leishmania (Viannia) braziliensis, and 40 paraffin-embedded biopsies of cutaneous lesions from dogs (...) with sporotrichosis, confirmed by isolation of Sporothrix schenckii in culture (control group), were studied.Immunohistochemistry was more sensitive in detecting amastigote forms than cytopathology and histopathology, with a positivity rate of 70% (n=28) versus 37.5% and 22.5% for histopathology and cytopathology, respectively. Cytoplasmic staining of mononuclear and endothelial cells was detected by IHC, which was highly specific since no cytoplasmic staining of these cells or staining of fungal structures

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2015 Transactions of the Royal Society of Tropical Medicine & Hygiene

191. Chronic Hand Infections. (PubMed)

, the paronychia or nail plate), subcutaneous (mainly lymphocutaneous sporotrichosis and dermatiaceous infections), and deep fungal infections. Each type of deep fungal infection has a "classic" presentation and this is emphasized. Finally, common chronic viral infections of the hand include warts and orf. Copyright © 2014 American Society for Surgery of the Hand. Published by Elsevier Inc. All rights reserved.

2014 Journal of Hand Surgery - American

192. Genome Sequence of the Pathogenic Fungus Sporothrix schenckii (ATCC 58251) (PubMed)

Genome Sequence of the Pathogenic Fungus Sporothrix schenckii (ATCC 58251) Sporothrix schenckii is a pathogenic dimorphic fungus that grows as a yeast and as mycelia. This species is the causative agent of sporotrichosis, typically a skin infection. We report the genome sequence of S. schenckii, which will facilitate the study of this fungus and of the Sporothrix schenckii group. Copyright © 2014 Cuomo et al.

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2014 Genome Announcements

193. Nocardiosis (Overview)

and often misdiagnosed as sporotrichosis (more common) because it also manifests as a primary lesion at the site of injury, followed by a proximal spread of lymphangitis and nodular lesions along the lymphatics. The lymphocutaneous or sporotrichoid form is the least common form of primary cutaneous nocardiosis. However, it probably is more common than reported, and diagnosis requires a high index of suspicion. Of 14 patients reported from 1920-1986, more than half had a history of gardening or thorn

2014 eMedicine.com

194. Hand Infections (Follow-up)

infection is sporotrichosis; this condition can appear with an ulcerative lesion, along with lymphadenopathy. Gardeners are most commonly infected. Oral itraconazole for 3-6 months is the current recommended course of treatment. Fungal abscesses or disseminated fungal infections can occur and are usually found in immunocompromised patients. [ ] Previous Next: Surgical Therapy As a rule, all abscess cavities must be drained. Antibiotics alone are not effective in treating pus. If the patient does

2014 eMedicine Surgery

195. Rhinosporidiosis (Diagnosis)

Principles and Practice of Infectious Diseases . 7th ed. Philadelphia, Pa: Churchill Livingstone Elsevier; 2010. 3365-3376. Hospenthal DR. Entomophthoramycosis, Lobomycosis, Rhinosporidiosis, and Sporotrichosis. Guerrant RL, Walker DH, Weller PF, eds. Tropical Infectious Diseases. Principles, Pathogens, & Practice . 3rd. Philadelphia, Pa: Saunders Elsevier; 2011. 603-607. Karunaratne WAE. Rhinosporidiosis in Man . London, England: The Athlone Press; 1964. Kwon-Chung KJ, Bennett JE. Rhinosporidiosis

2014 eMedicine.com

196. Cutaneous Manifestations of HIV Disease (Diagnosis)

the following: Scaling plaques Crusted ulcers Ecthymalike lesions Verrucous ulcers Inflammatory nodules Panniculitis Pustular lesions Draining sinuses Localized skin involvement resembling sporotrichosis is unusual. Primary cutaneous MAC infection manifesting as sporotrichosislike lesions was described in a patient with AIDS. [ ] The possibility of coinfections, which may be multiple, should be kept in mind. Coinfection with B quintana , MAC, and CMV has been reported in an AIDS patient. [ ] In patients

2014 eMedicine.com

197. Acute Febrile Neutrophilic Dermatosis (Follow-up)

, Gaudard S, Courtois JM, Dalac S, Collet E, Lambert D. [Sweet's syndrome and Yersinia enterocolitica infection]. Ann Dermatol Venereol . 1990. 117(11):858-60. . DiCaudo DJ, Ortiz KJ, Mengden SJ, Lim KK. Sweet syndrome (acute febrile neutrophilic dermatosis) associated with pulmonary coccidioidomycosis. Arch Dermatol . 2005 Jul. 141(7):881-4. . Freitas DF, Valle AC, Cuzzi T, et al. Sweet syndrome associated with sporotrichosis. Br J Dermatol . 2012 Jan. 166(1):212-3. . Oskay T, Karademir A, Kutluay L

2014 eMedicine.com

198. Acneiform Eruptions (Follow-up)

, Bagley MP, Khan Y, Schwartz RA. Pustular acneiform secondary syphilis. Cutis . 1986 Jan. 37(1):69-70. . Kusuhara M, Hachisuka H, Sasai Y. Statistical survey of 150 cases with sporotrichosis. Mycopathologia . 1988 May. 102(2):129-33. . Held JL, Andrew JE, Toback AC. Eruptive vellus hair cysts. Cutis . 1987 Sep. 40(3):259-60. . Fung MA, Berger TG. A prospective study of acute-onset steroid acne associated with administration of intravenous corticosteroids. Dermatology . 2000. 200(1):43-4. . Hurwitz RM

2014 eMedicine.com

199. Meningitis (Diagnosis)

, and plant products. Human infections are characteristically lymphocutaneous. Extracutaneous manifestations of sporotrichosis may occur, though meningeal sporotrichosis, which is the most severe form, is a rare complication. AIDS is a reported underlying risk factor in many described cases and is associated with a poor outcome. Infection with free-living amebas is an infrequent but often life-threatening human illness, even in immunocompetent individuals. N fowleri is the only species of Naegleria

2014 eMedicine.com

200. Lobomycosis (Diagnosis)

A, Restrepo A, Builes M, Robledo M. Lobomycosis. Report of the eighth Colombian case and review of the literature. J Cutan Pathol . 1976. 3(4):180-9. . Rodríguez G, Sarmiento L. The asteroid bodies of sporotrichosis. Am J Dermatopathol . 1998 Jun. 20(3):246-9. . Rodriguez G, Barrera GP. The asteroid body of lobomycosis. Mycopathologia . 1996-1997. 136(2):71-4. . Fischer M, Chrusciak Talhari A, Reinel D, Talhari S. [Sucessful treatment with clofazimine and itraconazole in a 46 year old patient after 32

2014 eMedicine.com

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