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Plaque

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34941. Identification of actin-, alpha-actinin-, and vinculin-containing plaques at the lateral membrane of epithelial cells Full Text available with Trip Pro

Identification of actin-, alpha-actinin-, and vinculin-containing plaques at the lateral membrane of epithelial cells In this paper, a new type of spot desmosome-like junction (type II plaque) is described that is scattered along the entire lateral plasma membrane of rat and human intestinal epithelium. Ultrastructurally type II plaques differed from the classical type of epithelial spot desmosome ("macula adherens", further denoted as type I desmosome) by weak electron density of the membrane (...) -associated plaque material, association of the plaques with microfilaments rather than intermediate filaments, and poorly visible material across the intercellular space. Thus, type II plaques resemble cross-sections of the zonula adherens. Immunofluorescence-microscopic studies were done using antibodies to a main protein associated with the plaques of type I desmosomes (desmoplakin I) and to the three major proteins located at the plaques of the zonula adherens (actin, alpha-actinin, and vinculin). Two

1986 The Journal of cell biology

34942. Forehead plaque: a presenting skin sign in tuberous sclerosis. Full Text available with Trip Pro

Forehead plaque: a presenting skin sign in tuberous sclerosis. The forehead plaque can be the earliest skin manifestation of tuberous sclerosis and its presence may lead to early diagnosis and appropriate genetic counselling.

1987 Archives of Disease in Childhood

34943. Plaque formation by virulent Shigella flexneri. Full Text available with Trip Pro

Plaque formation by virulent Shigella flexneri. An in vitro tissue culture plaque assay was developed to investigate the intracellular replication and intercellular spread of virulent shigellae. Shigella plaques were formed in HeLa cell monolayers in the presence of an agarose overlay containing tissue culture medium and gentamicin, which eliminated extracellular bacterial growth. Microscopically, the plaques were characterized by a central area of dead host cells surrounded by cells infected (...) with shigellae. Cells further away from the plaque center were uninfected. Inclusion of chloramphenicol or nalidixic acid in the overlay completely abolished plaque formation. Plaque formation was completely inhibited when infected monolayers were shifted from 37 to 30 degrees C. Shifting infected monolayers from 30 degrees C, where plaques do not form, to 37 degrees C resulted in the formation of plaques. Cultures of Shigella boydii, Shigella sonnei (form I), and all six serotypes of Shigella flexneri

1985 Infection and immunity

34944. Immunoperoxidase plaque staining for the detection of pseudorabies virus. Full Text available with Trip Pro

Immunoperoxidase plaque staining for the detection of pseudorabies virus. A quantitative indirect immunoperoxidase plaque staining method was developed for the detection of pseudorabies virus infection in a pig kidney cell line (PK-15). The method is rapid and specific and foci of infection, represented by stained plaques, are easily counted by the unaided eye. Possible modification of this technique in a plaque reduction assay for the detection of antipseudorabies virus antibody is also

1986 Canadian Journal of Veterinary Research

34945. Transforming gene in human atherosclerotic plaque DNA. Full Text available with Trip Pro

Transforming gene in human atherosclerotic plaque DNA. The monoclonal hypothesis equates atherosclerotic plaques with benign smooth muscle cell tumors and proposes that plaques can arise via mutational or viral events. Here, we provide direct evidence that molecular events, heretofore associated only with tumor cells, are common to plaque cells as well. Three distinct groups of human coronary artery plaque (hCAP) DNA samples transfected into NIH 3T3 cells gave rise to transformed foci. DNA (...) elicited tumors after injection into nude mice (6/42). Several distinct high molecular weight (greater than 6.6 kilobases) bands were detected after BamHI-digested tumor DNA was hybridized to Alu. Preliminary characterization of these hCAP DNA-associated tumors indicates that they are similar to the fibrosarcomas that arise after injection of ras-transformed cells into nude mice. We propose that transforming genes in plaque cells behave in a manner analogous to the way in which oncogenes behave

1986 Proceedings of the National Academy of Sciences of the United States of America

34946. Analysis by plaque reduction neutralization assay of intertypic rotaviruses suggests that gene reassortment occurs in vivo. Full Text available with Trip Pro

Analysis by plaque reduction neutralization assay of intertypic rotaviruses suggests that gene reassortment occurs in vivo. The SB-1A rotavirus recovered from a diarrheic piglet in the United States is a naturally occurring intertypic rotavirus. When studied by reciprocal neutralization tests, the SB-1A virus was similar, if not identical, to the porcine Gottfried virus (serotype 4) and the porcine OSU virus (serotype 5). Analysis of reassortant viruses prepared from the SB-1A virus

1987 Journal of clinical microbiology

34947. Butyrate and propionate: important components of toxic dental plaque extracts. Full Text available with Trip Pro

Butyrate and propionate: important components of toxic dental plaque extracts. Extracts of in vitro-cultured human dental plaque contain factors toxic to mammalian cells. Previous studies demonstrated that those toxic factors most readily released from cultured plaque had very low molecular weights and were heat stable. Studies reported here demonstrate that metabolic end products including short-chain fatty acids were present in fractions containing the low-molecular-weight, heat-stable (...) factors. The salts of two of these acids, butyrate and propionate, inhibited proliferation of both mouse L929 cells and human gingival fibroblasts. Furthermore, when tested at concentrations present in plaque extracts, the inhibitory effects of butyrate and propionate accounted for essentially all the inhibitory potential of the extracts. These findings, taken together with those of other groups, suggest that butyrate and propionate, end products of dental plaque metabolism, may have an etiological

1981 Infection and immunity

34948. Different modes of internalization of proteins associated with adhaerens junctions and desmosomes: experimental separation of lateral contacts induces endocytosis of desmosomal plaque material. Full Text available with Trip Pro

Different modes of internalization of proteins associated with adhaerens junctions and desmosomes: experimental separation of lateral contacts induces endocytosis of desmosomal plaque material. The distribution and fate of two junctional complexes, zonula adhaerens and desmosomes, after dissociation of cell-cell contacts is described in MDBK cells. Junctions were split between adjacent cells by treatment with EGTA and proteins associated with the plaques of zonulae adhaerentes and desmosomes (...) were localized by immunological methods. Splitting of these junctions is accompanied by the dislocation of desmosomal plaque protein from the cell periphery and its distribution in punctate arrays over the whole cytoplasm. By contrast, vinculin associated with zonulae adhaerentes is still seen at early times (0.5-1 h) in a conspicuous belt-like structure which, however, is displaced from the plasma membrane. Strong vinculin staining is maintained on leading edges of free cell surfaces. Electron

1982 The EMBO journal

34949. Cell surface hydrophobicity of dental plaque microorganisms in situ. Full Text available with Trip Pro

Cell surface hydrophobicity of dental plaque microorganisms in situ. The cell surface hydrophobicity of bacteria obtained directly from human tooth surfaces was assayed by measuring their adherence to liquid hydrocarbons. Fresh samples of supragingival dental plaque were washed and dispersed in buffer. Adherence of the plaque microorganisms to hexadecane, octane, and xylene was tested turbidimetrically and by direct microscopic observation. The results clearly show that the vast majority (...) of bacteria comprising dental plaque exhibit pronounced cell surface hydrophobicity. These data support the hypothesis that hydrophobic interactions play a major role in mediating bacterial adherence on tooth surfaces.

1983 Infection and immunity

34950. Plaques and tangles and transmitter deficiencies in dementia. Full Text available with Trip Pro

Plaques and tangles and transmitter deficiencies in dementia. 6214616 1982 12 02 2018 11 13 0022-3050 45 6 1982 Jun Journal of neurology, neurosurgery, and psychiatry J. Neurol. Neurosurg. Psychiatry Plaques and tangles and transmitter deficiencies in dementia. 563-4 Mann D M DM Yates P O PO Hawkes J J eng Journal Article England J Neurol Neurosurg Psychiatry 2985191R 0022-3050 X4W3ENH1CV Norepinephrine IM Adult Aged Alzheimer Disease pathology Brain Injuries pathology Dementia pathology Down

1982 Journal of neurology, neurosurgery, and psychiatry

34951. Immune response mediated by liposome-associated protein antigens. I. Potentiation of the plaque-forming cell response. Full Text available with Trip Pro

Immune response mediated by liposome-associated protein antigens. I. Potentiation of the plaque-forming cell response. Mice, immunized with liposome-associated bovine serum albumin (LSM-BSA), showed a significantly higher BSA-specific plaque-forming cell (PFC) response than did mice injected with fluid BSA (fBSA). Physical association between the liposome carrier and the protein antigen is imperative for potentiating the PFC response, since the injection of empty liposomes, together with fBSA

1982 Immunology

34952. Spontaneous plaque forming cells in the peripheral blood of patients with systemic lupus erythematosus. Full Text available with Trip Pro

Spontaneous plaque forming cells in the peripheral blood of patients with systemic lupus erythematosus. A reverse haemolytic plaque assay using staphylococcal protein A coupled to sheep red blood cells was set up in Cunningham chambers. Using this method, the numbers of Ficoll-Hypaque isolated peripheral blood lymphocytes (PBL) secreting IgG, IgA or IgM without preceding culture or mitogen stimulation were estimated in patients with systemic lupus erythematosus (SLE) and control subjects. Seven (...) patients with clinically inactive SLE at the time of the study had values similar to those of the control subjects. In contrast, eight patients who had clinically active SLE had markedly increased numbers of PBL secreting IgG, IgA and IgM. Control experiments confirmed that the plaques were due to Ig secretion by lymphoid cells rather than to immune complexes adsorbed onto Fc receptor bearing cells or to passively adsorbed Ig. The results confirm the expected polyclonal B cell activation in patients

1982 Clinical and experimental immunology

34953. Biological properties of a plaque-inducing agent obtained from Acholeplasma oculi. Full Text available with Trip Pro

Biological properties of a plaque-inducing agent obtained from Acholeplasma oculi. A plaque-forming agent arose spontaneously during cloning of Acholeplasma oculi 19L. The agent produced plaques on A. oculi 19L and A. oculi-i, but not on A. laidlawii, A. modicum, or wild isolates of A. oculi. The agent required horse serum for plaque formation as well as for adsorption to the indicator lawn; however, it was extremely sensitive to an inhibitor in some horse sera. The agent retained infectivity

1983 The Yale journal of biology and medicine

34954. Small erythematous mucosal plaques: an endoscopic sign of Crohn's disease. Full Text available with Trip Pro

Small erythematous mucosal plaques: an endoscopic sign of Crohn's disease. A non-ulcerated granulomatous lesion of the large bowel mucosa has been found in 11 patients, nine of whom already had or eventually developed classical features of Crohn's disease. These lesions, which are multiple, consist of small well-circumscribed raised erythematous plaques surrounded by normal mucosa. At biopsy there is focal haemorrhage of the lamina propria, rupture of the crypts, release of mucus, and frank

1980 Gut

34955. Clinical evaluation of B cell and T-regulator cell function using a protein A haemolytic plaque assay. Full Text available with Trip Pro

Clinical evaluation of B cell and T-regulator cell function using a protein A haemolytic plaque assay. Using the protein A haemolytic plaque assay (PrA-HPA) and standardized, optimal conditions, pokeweed mitogen (PWM) stimulated peripheral blood lymphocytes (PBL) from normal donors produced 5,500-48,000 (mean 16,955) IgG plaque-forming cells (PFC) per 10(6) lymphocytes, 3,375-38,000 (mean 14,179) IgA-PFC/10(6) and 5,500-69,750 (mean 18,684) IgM-PFC/10(6). Addition of 4 micrograms/ml

1981 Clinical and experimental immunology

34956. Size-variant pp60src proteins of recovered avian sarcoma viruses interact with adhesion plaques as peripheral membrane proteins: effects on cell transformation. Full Text available with Trip Pro

Size-variant pp60src proteins of recovered avian sarcoma viruses interact with adhesion plaques as peripheral membrane proteins: effects on cell transformation. We have shown previously that the membrane association of the src proteins of recovered avian sarcoma viruses (rASVs) 1702 (56 kilodaltons) and 157 (62.5 kilodaltons), whose size variations occur within 8 kilodaltons of the amino terminus, is salt sensitive and that, in isotonic salt, these src proteins fractionate as soluble (...) sedimentation of extracts from cells infected either with rASV 1702 or rASV 157 showed that soluble src proteins of these viruses were distributed between free and complexed forms as has been demonstrated for wild-type Rous sarcoma virus pp60src. Pulse-chase studies with rASV pp60src showed that, like wild-type Rous sarcoma virus pp60src, it was transiently found in a complexed form. Indirect immunofluorescence showed that size-variant pp60src proteins are localized in adhesion plaques and regions of cell

1984 Molecular and cellular biology

34957. Significance of two desmosome plaque-associated polypeptides of molecular weights 75 000 and 83 000. Full Text available with Trip Pro

Significance of two desmosome plaque-associated polypeptides of molecular weights 75 000 and 83 000. Isolated desmosomes from bovine epidermis contain two major polypeptides of mol. wts. 75 000 (D6) and 83 000 (D5) which, like the desmoplakins of mol. wt. greater than 200 000, are associated with the insoluble desmosomal plaque structure. We have characterized these two polypeptides and examined their significance by peptide map comparisons and translation of bovine epidermal mRNA in vitro

1983 The EMBO journal

34958. Specific attachment of desmin filaments to desmosomal plaques in cardiac myocytes. Full Text available with Trip Pro

Specific attachment of desmin filaments to desmosomal plaques in cardiac myocytes. Intercellular junctions which are similar in ultrastructure and protein composition to typical desmosomes have so far only been found in epithelial cells and in heart tissue, specifically in the intercalated disks of cardiac myocytes and at cell boundaries between Purkinje fiber cells. In epithelial cells the cytoplasmic side of desmosomes, the 'desmosomal plaque', represents a specific attachment structure (...) for the anchorage of intermediate filaments (IF) of the cytokeratin type. Cardiac myocytes do not contain cytokeratin filaments. In primary cultures of rat cardiac myocytes, we have examined by immunofluorescence and electron microscopy, using single and double label techniques, whether other types of IF are attached to the desmosomal plaques of the heart. Antibodies to desmoplakin, the major protein of the desmosomal plaque, have been used to label specifically the desmosomal plaques. It is shown

1983 The EMBO journal

34959. Plaquing procedure for infectious hematopoietic necrosis virus. Full Text available with Trip Pro

Plaquing procedure for infectious hematopoietic necrosis virus. A single overlay plaque assay was designed and evaluated for infectious hematopoietic necrosis virus. Epithelioma papillosum carpio cells were grown in normal atmosphere with tris(hydroxymethyl)aminomethane- or HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid)-buffered media. Plaques were larger and formed more quickly on 1- to 3-day-old cell monolayers than on older monolayers. Cell culture medium with a 10% addition (...) of fetal calf serum (MEM 10) or without serum (MEM 0) were the most efficient virus diluents. Dilution with phosphate-buffered saline, saline, normal broth, or deionized water reduced plaque numbers. Variations in the pH (7.0 to 8.0) of a MEM 0 diluent did not affect plaque numbers. Increasing the volume of viral inoculum above 0.15 ml (15- by 60-mm plate) decreased plaquing efficiency. Significantly more plaques occurred under gum tragacanth and methylcellulose than under agar or agarose overlays

1980 Applied and environmental microbiology

34960. Aminomalonic acid: identification in Escherichia coli and atherosclerotic plaque. Full Text available with Trip Pro

Aminomalonic acid: identification in Escherichia coli and atherosclerotic plaque. Aminomalonic acid (Ama) has been isolated from proteins of Escherichia coli and human atherosclerotic plaque. The presence of Ama has important biological implications because the malonic acid moiety potentially imparts calcium binding properties to protein. Ama was obtained by anaerobic alkaline hydrolysis and identified by chromatographic behavior, quantitative acid-mediated decarboxylation to glycine (...) , and unambiguous gas chromatographic/mass spectral detection. The chromatographic, chemical, and mass spectral properties of naturally occurring Ama were identical to those of the synthetic compound. Amino acid analysis and GC/mass spectrometry also revealed the presence of beta-carboxyaspartic acid and gamma-carboxyglutamic acid in the base hydrolysate of human atherosclerotic plaque. The ratio of Ama to beta-carboxyaspartic acid to gamma-carboxyglutamic acid was 20:1:10, and the quantity of Ama per 1,000

1984 Proceedings of the National Academy of Sciences of the United States of America

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