How to Trip Rapid Review

Step 1: Select articles relevant to your search (remember the system is only optimised for single intervention studies)

Step 2: press

Step 3: review the result, and maybe amend the or if you know better! If we're unsure of the overall sentiment of the trial we will display the conclusion under the article title. We then require you to tell us what the correct sentiment is.

1,281 results for

Cholesteatoma

by
...
Latest & greatest
Alerts

Export results

Use check boxes to select individual results below

SmartSearch available

Trip's SmartSearch engine has discovered connected searches & results. Click to show

1261. Chorda tympani neuroma masquerading as cholesteatoma. Full Text available with Trip Pro

Chorda tympani neuroma masquerading as cholesteatoma. Facial nerve neuromas occur throughout the course of the facial nerve and its branches, however lesions occurring on the chorda tympani branch are exceptionally rare. We present a case where the diagnosis was made intra-operatively; the patient was pre-operatively thought to have had a cholesteatoma. Total resection is the treatment of choice for these cases. Early diagnosis, aided by high resolution computed tomography (CT) scanning

2003 Journal of Laryngology & Otology

1262. Aggressiveness and quantification of epithelial proliferation of middle ear cholesteatoma by MIB1. (Abstract)

Aggressiveness and quantification of epithelial proliferation of middle ear cholesteatoma by MIB1. To assess an easy method that predicts cholesteatoma aggressiveness.An experimental prospective study.Monoclonal antibody MIB1 was used to determine epithelium proliferation in 91 cholesteatomatous ears. Clinical and surgical parameters were compared with proliferation activity to determine pathological and clinical correlation.Statistical correlations were established between hyperproliferation (...) of the cholesteatoma and severe bone erosion (leading to major cholesteatoma complications) and between hyperproliferation and middle ear inflammation (associated with more surgical difficulties and a higher risk of recurrence). A high proliferation index was also found in children's cholesteatoma, which is known to have more aggressive behavior.Immunohistochemical use of the MIB1 antibody is a simple technique that can help to determine the aggressiveness of a cholesteatoma.

2003 Laryngoscope

1263. Expression of a cathelicidin antimicrobial peptide is augmented in cholesteatoma. (Abstract)

Expression of a cathelicidin antimicrobial peptide is augmented in cholesteatoma. Antimicrobial peptides are active defense components of innate immunity. Their importance was confirmed at epithelial surfaces as immediate barrier effectors in preventing infection. Cathelicidins are peptide antibiotics that are receiving increasing attention. Several studies have shown that overexpression of cathelicidin results in augmented protection against bacterial infection and prevention of local (...) infection and systemic invasion of microbes. The goal of the study was to investigate whether cathelicidin is upregulated in cholesteatoma epithelium compared with normal skin.Twenty patients from a prospective study of cholesteatoma tissues and normal skins were enrolled in the study. The specimens were divided into two portions. One portion was used for subsequent RNA studies; the other was used for immunohistochemical staining.Reverse transcriptase-polymerase chain reaction was used to assess

2003 Laryngoscope

1264. Management of cholesteatoma: status of the canal wall. (Abstract)

Management of cholesteatoma: status of the canal wall. Management of chronic otitis media with cholesteatoma remains controversial. The purpose of the study is to examine factors associated with the surgical approach to manage cholesteatoma.A retrospective review.A retrospective review was made of all primary cases of mastoid surgery for cholesteatoma performed at an otological center between 1995 and 2000. During the study period, 486 ears underwent surgery for cholesteatoma. Data included (...) procedures performed, location and extent of the disease, residual and recurrent disease, complications, reasons for staging the surgery, and duration of follow-up.The canal wall remained intact in 68.5% of ears. The majority of the remainder of the patients underwent a canal wall down technique with mastoid obliteration. Residual cholesteatoma was found in 26.9% of second procedures and in 2.7% of third procedures.The majority of patients with cholesteatoma can be adequately managed with a canal intact

2003 Laryngoscope

1265. Osteoclast stimulating and differentiating factors in human cholesteatoma. (Abstract)

Osteoclast stimulating and differentiating factors in human cholesteatoma. To investigate the expression of osteoclast-activating and differentiating factors and to study the occurrence of osteoclast precursor cells and osteoclasts in acquired human cholesteatoma tissue.We examined 21 cholesteatoma samples versus 18 normal auditory canal skin specimens for the expression of osteoprotegerin ligand (OPGL), osteoprotegerin (OPG), and macrophage-colony stimulating factor (M-CSF) using reverse (...) transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry. Immunohistochemistry and computer-assisted microscopy using markers CD4, CD11a, CD11b, CD14, CD51, CD68, and TRAP obtained the detection of osteoclast cell lineage.An increased expression of the investigated cytokines M-CSF, OPG, and OPGL was demonstrated by immunohistochemistry and RT-PCR in cholesteatoma tissue compared with normal external meatal skin. Several CD4-positive cells exhibited a co-expression for OPGL within

2003 Laryngoscope

1266. Evidence against neonatal aspiration of keratinizing epithelium as a cause of congenital cholesteatoma. (Abstract)

Evidence against neonatal aspiration of keratinizing epithelium as a cause of congenital cholesteatoma. It has been suggested that congenital cholesteatoma may be caused by perinatal aspiration of squamous epithelium.Microscopic study of fetal temporal bones.Thirty-one temporal bones from infants who died of sudden infant death syndrome before 1 year of age and 27 temporal bones obtained from preterm fetal deaths aged 4 to 8 months of fetal development were studied to assess signs of aspiration (...) of squamous epithelium in the middle ear.None of the prenatal or postnatal temporal bones showed keratinizing epithelial cells or lanugo. A certain number of specimens displayed a nonspecific inflammatory lymphocytic infiltration.The data in the present study do not support the theory of amniotic fluid and squamous epithelial aspiration as an origin of congenital cholesteatoma.

2003 Laryngoscope

1267. Value of ear endoscopy in cholesteatoma surgery. (Abstract)

Value of ear endoscopy in cholesteatoma surgery. The purpose of this study was to assess the value of ear endoscopy in cholesteatoma surgery and to demonstrate its consequence in improving surgical outcome.A total of 92 ears with acquired cholesteatoma (primary or secondary) were operated on. In this prospective study, 82 cases were operated on by using canal wall up (CWU) technique, and 10 cases were on operated on by using canal wall down (CWD) procedure. Endoscopically guided ear surgery (...) in both CWU and CWD groups, followed by the facial recess and the undersurface of the scutum in the CWU cases. Reconstruction of the hearing mechanism was performed in the primary surgery in 86 cases (93.5%) and postponed to the second stage in only six cases (6.5%). Out of the 82 CWU cases, 35 second-look endoscopic explorations (42.7%) were performed. Three recurrences (8.6%) were identified. Two cases showed a tiny residual cholesteatoma pearl, and the third showed a larger open residual

2002 Otology and Neurotology

1268. Expression of epidermal growth factor, tumor necrosis factor-alpha, and interleukin-1alpha in chronic otitis media with or without cholesteatoma. (Abstract)

Expression of epidermal growth factor, tumor necrosis factor-alpha, and interleukin-1alpha in chronic otitis media with or without cholesteatoma. The object of this study was to compare the expression of epidermal growth factor, interleukin-1alpha, and tumor necrosis factor-alpha in chronic otitis media with or without cholesteatoma.It has been reported that cytokines and epidermal growth factor are effective in the bone resorption process in chronic otitis media. Bone resorption can also occur (...) in chronic otitis media without cholesteatoma. However, comparative analysis is lacking. This issue has been investigated in a blind, controlled and prospective analysis.The activities of interleukin-1alpha, tumor necrosis factor-alpha, and epidermal growth factor were determined by commercially available enzyme-linked immunosorbent assay kits in tissue biopsy samples from 16 patients without cholesteatoma and from 23 patients with cholesteatoma (cholesteatoma epithelium). To establish a control group

2002 Otology and Neurotology

1269. Telomerase activity, telomere length, and apoptosis: a comparison between acquired cholesteatoma and squamous cell carcinoma. (Abstract)

Telomerase activity, telomere length, and apoptosis: a comparison between acquired cholesteatoma and squamous cell carcinoma. Cholesteatoma disease is characterized by accumulation of keratinizing epithelium. Several molecular markers of tumor formation have been found in cholesteatoma (e.g. upregulation of matrix metalloproteinases, c and activation of angiogenesis). Other molecular findings clearly distinguish between cholesteatoma and malignant tumors (e.g., lack of chromosomal instability (...) , intact checkpoint responses). To further distinguish the molecular mechanisms in cholesteatoma from malignant tumors, the authors determined telomerase activity and telomere length in both tissue types.To evaluate the role of telomerase activation and telomere length in cholesteatoma, 29 cholesteatoma samples and 9 squamous cell carcinomas were analyzed for telomerase activity and telomere length. In addition, the rate of apoptosis was determined in both groups, using the TdT-mediated dUTP nick end

2002 Otology and Neurotology

1270. Cartilage palisade tympanoplasty in sinus and tensa retraction cholesteatoma. (Abstract)

Cartilage palisade tympanoplasty in sinus and tensa retraction cholesteatoma. The aim of this study was to investigate whether reconstruction of the eardrum with palisade cartilage technique could prevent retraction of the new eardrum after surgery for sinus and tensa retraction cholesteatoma in children and to investigate the postoperative hearing.In 32 children aged 5 to 15 years, operated on from June 1995 to October 2000 for cholesteatoma (21 with sinus cholesteatoma and 11 with tensa (...) retraction cholesteatoma) the eardrum was reconstructed with the palisade cartilage technique. Postoperatively, the children were seen as outpatients and were recently reevaluated with otomicroscopy, tympanometry, and audiometry. All patients (100%) were reevaluated on an average of 37 months (range 3-63 months).Postoperative retractions, perforations, cholesteatoma recurrence, and hearing.At the final examination, posterosuperior retraction was observed in two patients, both operated on for sinus

2002 Otology and Neurotology

1271. Increased proliferation and migration of epithelium in advancing experimental cholesteatomas. (Abstract)

Increased proliferation and migration of epithelium in advancing experimental cholesteatomas. Hyperproliferative and migratory process of keratinocytes are part of the pathogenesis of cholesteatoma.Cytokeratin (CK) changes were prominent in the most rapidly expanding regions of cholesteatoma formation.The three types of animal model-canal ligation (CL), retraction pocket (RP), and propylene glycol (PG)-were induced in Mongolian gerbils. The monoclonal antibodies to CK1/10, CK5/6, and CK13/16 (...) were used for immunohistochemistry. The intensity of immunostaining in the pars tensa of the tympanic membrane was measured using the densitometry and compared with respect to the stage of cholesteatoma and the type of animal model.With cholesteatoma formation, CK expressions were significantly increased at the peripheral part of the pars tensa, the expanding part of cholesteatoma. Among the CKs tested, the prominent changes were observed in expression of CK13/16, a marker for hyperproliferation

2002 Otology and Neurotology

1272. Detection of Chlamydia pneumoniae in cholesteatoma tissue: any pathogenetic role? (Abstract)

Detection of Chlamydia pneumoniae in cholesteatoma tissue: any pathogenetic role? Acquired cholesteatoma is a complication of chronic otitis media that is usually associated with an intense local inflammatory reaction. Cholesteatoma probably arises from epithelial migration close to an ongoing host inflammatory response attributable to a chronic bacterial infection. Chlamydia pneumoniae is an intracellular microorganism associated with several pathologic conditions originally considered (...) noninflammatory, including asthma, atherosclerosis, and Alzheimer disease. To investigate a possible relationship between C. pneumoniae and the development of cholesteatoma, tissue was studied in three different layers by polymerase chain reaction analysis. The results were compared with those relative to other two common middle-ear pathogens, Mycoplasma pneumoniae and Haemophilus influenzae.Cholesteatoma specimens were collected from 32 patients undergoing middle ear surgery. A series of 5 microm-thick

2003 Otology and Neurotology

1273. Expression of hypoxia inducible factor 1 alpha and Von Hippel Lindau protein in human middle ear cholesteatoma. (Abstract)

Expression of hypoxia inducible factor 1 alpha and Von Hippel Lindau protein in human middle ear cholesteatoma. To evaluate the role of hypoxia in cholesteatoma progression.Immunohistochemical analysis of paraffin-embedded human specimens.Thirteen middle ear cholesteatomas and 10 samples of normal human external ear canal skin were stained immunohistochemically for the presence of hypoxia inducible factor 1 alpha (HIF 1 alpha) and Von Hippel Lindau protein. Specimens were then analyzed (...) semiquantitatively.Staining for both antibodies could be detected in all cholesteatomas (perimatrix and matrix), as well as in the samples of normal human ear canal skin. Cholesteatoma specimens showed statistically significant increased staining when compared with normal human skin and mucosa. The age of patients and relapse surgery affected immunohistochemical staining of HIF 1 alpha and Von Hippel Lindau protein.Elevated staining intensities for HIF 1 alpha and Von Hippel Lindau protein in cholesteatoma tissue

2003 Laryngoscope

1274. A tympanometric comparison of tympanoplasty with cartilage palisades or fascia after surgery for tensa cholesteatoma in children. (Abstract)

A tympanometric comparison of tympanoplasty with cartilage palisades or fascia after surgery for tensa cholesteatoma in children. The aim of the present study is to compare the tympanometric and functional findings of cartilage palisade tympanoplasty with those of tympanoplasty with temporalis fascia grafting after one-stage surgery in children with tensa cholesteatoma.In children aged 5 to 15 years (mean, 9.5 years) with tensa cholesteatoma, cartilage palisade tympanoplasty was performed in 32 (...) ears and fascia tympanoplasty in 29 ears. Tympanometry was performed in 31 ears with cartilage palisade tympanoplasty (the palisade group) and 28 ears with fascia tympanoplasty (the fascia group) at follow-up a median of 48 months after surgical removal of the cholesteatoma (range, 3-75 months).Postoperative perforations, tympanometric parameters (tympanogram type, compliance, tympanometric width), and hearing.All postoperative re-perforations occurred in the fascia group. However

2003 Laryngoscope

1275. A murine model of cholesteatoma-induced bone resorption using autologous dermal implantation. (Abstract)

A murine model of cholesteatoma-induced bone resorption using autologous dermal implantation. To investigate a novel murine model for dermal implant-induced osteolysis analogous to bone resorption observed in middle ear cholesteatoma.Animal experiment.We placed autologous dermal implants on the surface of mouse calvaria. The calvaria were examined at days 1, 3, 5, 7, and 14 after implantation by histological study and tartrate-resistant acid phosphatase immunohistochemical processing to detect (...) resorption related to cholesteatoma.

2003 Laryngoscope

1276. Gene expression analysis of human middle ear cholesteatoma using complementary DNA arrays. (Abstract)

Gene expression analysis of human middle ear cholesteatoma using complementary DNA arrays. To identify genes regulated in human cholesteatoma compared with normal skin tissue using complementary DNA arrays.In vitro analysis.Eight cholesteatoma and retroauricular skin samples were obtained from the same patients during surgery. Upregulated and downregulated genes were highlighted using complementary DNA arrays for screening. Reverse transcriptase-polymerase chain reaction and immunohistochemical (...) staining were performed to confirm the results of the complementary DNA array.Twelve genes were found to be induced or upregulated in cholesteatoma compared with skin samples. These included genes involved in cell proliferation and differentiation (eg, calgranulin A, calgranulin B, psoriasin, thymosin beta-10) and cell invasion (eg, cathepsin C, cathepsin D, cathepsin H). Analyses by means of reverse transcription-polymerase chain reaction showed enhanced expression of several genes including

2003 Laryngoscope

1277. Autograft ossiculoplasty in cholesteatoma surgery: is it feasible? Full Text available with Trip Pro

Autograft ossiculoplasty in cholesteatoma surgery: is it feasible? To investigate whether autologous ossicles can be safely used in ossicular reconstruction in cholesteatoma surgery after attempting cholesteatoma removal under the operating microscope.A prospective fine-section histological study of formalin-stored ossicles, harvested from cholesteatomatous ears, to evaluate for existence of residual cholesteatoma after surface disease clearance under the operating microscope.One hundred four (...) ossicles were harvested from 76 patients with cholesteatoma for the study. These malleus heads and includes were categorized into three groups: group 1, ossicles with retained shape and useful bulk, treated by microscopic stripping alone; group 2, ossicles with retained shape and useful bulk, treated by microscopic stripping and drilling; and group 3, badly eroded ossicles, treated by microscopic stripping alone. These treated ossicles were then subjected to 4 microm histopathological study.Residual

2003 Laryngoscope

1278. Reversible canal wall down mastoidectomy for acquired cholesteatomas: preliminary results. (Abstract)

Reversible canal wall down mastoidectomy for acquired cholesteatomas: preliminary results. The educational objectives were to discuss a new technique to manage acquired cholesteatomas and to compare this with standard approaches used for cholesteatoma removal. The study objective was to determine the feasibility of using a new approach, the reversible canal wall down approach, in conjunction with bone cement to remove acquired cholesteatomas and, potentially, preserve the ossicular (...) chain.Retrospective review of five patients who underwent resection of their acquired cholesteatomas using a technique that maximizes exposure, yet preserves the posterior canal wall and, potentially, the ossicular chain.In the setting of a tertiary otological referral center, five consecutive patients with acquired cholesteatomas underwent reversible canal wall down surgery. Four patients were male (ages, 41, 53, 26, and 10 y), and one patient was a woman [age, 51 y]). A reversible canal wall down mastoidectomy

2003 Laryngoscope

1279. Immunohistochemical investigations on external auditory canal cholesteatomas. (Abstract)

Immunohistochemical investigations on external auditory canal cholesteatomas. The aim of the study was to analyze the biological behavior of external auditory canal cholesteatoma (EACC). The expression and distribution of relevant markers such as transforming growth factor-alpha (TGF-alpha), epidermal growth factor-receptor (EGFR), and the proliferation marker MIB 1 were studied in comparison with normal auditory meatal skin.EACC are uncommon and knowledge concerning etiology and pathogenesis (...) in cholesteatoma was significantly higher than in normal external auditory skin. Cholesteatoma epithelium also showed an enhanced expression of TGF-alpha and EGFR. Inflammatory infiltrate was observed in the perimatrix to various degrees.These results suggest that similar to the middle ear cholesteatoma, a chronic inflammatory process underlies the EACC, and the inflammatory stimuli may alter keratinocyte proliferation.

2003 Otology and Neurotology

1280. Lipopolysaccharide concentration and bone resorption in cholesteatoma. (Abstract)

Lipopolysaccharide concentration and bone resorption in cholesteatoma. There is a relationship between the local lipopolysaccharide (LPS) concentration in cholesteatoma and local bone resorption in chronic otitis media (COM) with cholesteatoma.During the past decade, it has become known that the recruitment of osteoclasts is the main causative factor that induces bone destruction in COM with cholesteatoma. Cellular inflammation factors like cytokines may trigger the osteoclast. Sequel (...) to this, LPS is able to up-regulate cytokines. This makes it of interest to study whether the local LPS concentration is related to bone resorption in cholesteatoma.Twenty-four cholesteatoma samples and control tissue from COM patients without cholesteatoma were collected. During surgery, the degree of bone resorption was established and classified. Retrospectively, the authors checked whether patients had chronic purulent otorrhea. LPS concentration of the tissue samples was measured by the limulus

2003 Otology and Neurotology

To help you find the content you need quickly, you can filter your results via the categories on the right-hand side >>>>