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Blood Collection Tube

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161. Blood sampling, neonatal capillary

the following equipment ( ): gloves and aprons ( ) cotton wool or gauze capillary tube and/or blood bottle Tenderfoot® or other age/size appropriate automated incision device ( ) clean tray to hold equipment sharps disposal box ( ) Selecting an appropriate device An appropriately sized and age-appropriate automated incision device (eg Tenderfoot® designed specifically for use on neonates should be selected ). Skin puncture must be no deeper than 2mm for term neonates. A penetrative depth of no more than 1mm (...) ( Tenderfoot® Heel Incision Device) . There is no need to discard the first drop - the first drop of blood can be used if the baby’s heel has been cleaned thoroughly ( ). While maintaining grip, hold the heel so that blood is allowed to hang downwards ( ). Gently but firmly compress the baby’s heel to form a large droplet of blood ( ). Do not squeeze the foot in an attempt to increase blood flow ( ). Hold the capillary tube or blood bottle to the blood droplet and touch ( ). See figure 4 below ( Tenderfoot

2014 Publication 1593

162. Point-of-Care faecal occult blood testing

any studies evaluating the diagnostic accuracy of any of these kits. Table 1: CE marked point of care faecal immunochemical test kits marketed for home use in the UK. Kit Manufacturer / Distributer. Method Hb threshold Price SELF- S U R E ™ Epitope Diagnostics Inc, San Diego, USA. (distributed by Personal Diagnostics, UK) Plastic stool collection device stabs stool at “two or more sites”, is then twisted into sealed sampling tube containing developing suspension and shaken. Test strip is screwed (...) into other end of sealed sampling tube and result given. Not specified £11.99p FOBCHECK © NanoRepro, Marburg, Germany. Distributed through Amazon.co.uk Plastic stool collection device stabs stool at ”various locations”, is then twisted into sealed sampling tube containing developing suspension and mixed. Top is unscrewed and 3 drops are dropped into the sample well of the test device and result given. 40 µg/l £15.95p (2 kits) Certain Bowel Health Test KOROGLU MEDICAL DEVICES LTD, Turkey. Distributed

2014 Publication 4878904

163. Practice Guidelines for Perioperative Blood Management

in advance ( e.g. , several days to weeks) to allow for proper patient preparation. Preadmission Patient Preparation Preadmission patient preparation includes (1) treatment of anemia, (2) discontinuation of anticoagulants and antiplatelet agents, and (3) preadmission autologous blood collection. Treatment of Anemia. The World Health Organization identifies anemia as hemoglobin thresholds of 11.0 g/dl for children 0.50–4.99 yr, 11.5 g/dl for children 5.0–11.99 yr, 12.0 g/dl for children 12.0–14.99 yr (...) Practice Guidelines for Perioperative Blood Management Practice Guidelines for Perioperative Blood Management:An Updated Report by the American Society of Anesthesiologists Task Force on Perioperative Blood Management* | Anesthesiology | ASA Publications 425070596 ASA Publications Log in to access full content You must be logged in to access this feature. ASA members enjoy complimentary access to ASA publications, as well as a variety of educational resources. Non-ASA Members Login Free

2015 American Society of Anesthesiologists

164. Patient blood management guidelines module 5. Obstetrics and maternity

Patient blood management guidelines module 5. Obstetrics and maternity Obstetrics and Maternity Patient Blood Management Guidelines: Module 5 Patient Blood Management Guidelines: Module 5 Obstetrics and MaternityPatient Blood Management Guidelines: Module 5 | Obstetrics and Maternity © National Blood Authority, 2015. With the exception of any logos and registered trademarks, and where otherwise noted, all material presented in this document is provided under a Creative Commons Attribution (...) -NonCommercial-ShareAlike 3.0 Australia (http://creativecommons.org/licenses/by-nc-sa/3.0/au/) licence. You are free to copy, communicate and adapt the work for noncommercial purposes, as long as you attribute the authors and distribute any derivative work (i.e. new work based on this work) only under this licence. If you adapt this work in any way or include it in a collection, and publish, distribute or otherwise disseminate that adaptation or collection to the public, it should be attributed

2015 Clinical Practice Guidelines Portal

165. Interventions to reduce wrong blood in tube errors in transfusion: a systematic review. (Abstract)

Interventions to reduce wrong blood in tube errors in transfusion: a systematic review. This systematic review addresses the issue of wrong blood in tube (WBIT). The objective was to identify interventions that have been implemented and the effectiveness of these interventions to reduce WBIT incidence in red blood cell transfusion. Eligible articles were identified through a comprehensive search of The Cochrane Library, MEDLINE, EMBASE, Cinahl, BNID, and the Transfusion Evidence Library (...) , for example, changes to blood sample labeling, weekly feedback, handwritten transfusion requests, and an electronic transfusion system. Four studies reported multiple interventions including education, second check of ID at sampling, and confirmatory sampling. It was not clear which intervention was the most effective. Sustainability of the effectiveness of interventions was also unclear. Targeted interventions, either single or multiple, can lead to a reduction in WBIT; but the sustainability

2013 Transfusion medicine reviews

166. Reduction of gross hemolysis in catheter-drawn blood using Greiner Holdex tube holder. Full Text available with Trip Pro

Reduction of gross hemolysis in catheter-drawn blood using Greiner Holdex tube holder. Blood collection through intravenous lines frequently causes spurious hemolysis. Due to specific structure, the tube holder Holdex (Greiner Bio-One GmbH, Kremsmuenster, Austria) is supposed to prevent erythrocyte injury in samples collected from catheters, so that we planned a specific study to support this hypothesis.Blood was collected from emergency department (ED) patients with 20-gauge catheter (...) . In patients with odd order numbers, first and second tubes were collected with conventional holder (BD Vacutainer One Use Holder, Becton Dickinson, Milan, Italy) and the third with Holdex, whereas in even patients first and second tubes were drawn with Holdex and the third using BD Vacutainer One Use Holder. The first tube was discarded, whereas the second and third were centrifuged and serum was tested for potassium, lactate dehydrogenase (LD) and hemolysis index.The final study population consisted

2013 Biochemia medica Controlled trial quality: uncertain

167. Effect of intraparenchymal blood patch on rates of pneumothorax and pneumothorax requiring chest tube placement after percutaneous lung biopsy. (Abstract)

collected included development of pneumothorax and placement of a chest tube.The rate of pneumothorax was reduced from 35% to 26% (p = 0.12) with the use of the blood patch, but the reduction was not significant. The rate of pneumothorax requiring chest tube placement was significantly reduced from 18% to 9% (p = 0.048). There was a greater benefit in the blood patch group when a 19-gauge guiding needle was used: Pneumothorax requiring chest tube placement was reduced from 19% to 3% whereas an increase (...) Effect of intraparenchymal blood patch on rates of pneumothorax and pneumothorax requiring chest tube placement after percutaneous lung biopsy. The purpose of this study was to determine whether an autologous intraparenchymal blood patch reduces the rate of pneumothorax and the rate of pneumothorax requiring chest tube placement after percutaneous lung biopsy.A prospective randomized controlled trial enrolling 242 patients was conducted. Adult patients undergoing percutaneous biopsy of lung

2013 AJR. American journal of roentgenology Controlled trial quality: uncertain

168. Association of postoperative blood pressure and bleeding after cardiac surgery. (Abstract)

Association of postoperative blood pressure and bleeding after cardiac surgery. We sought to characterize the relationship between postoperative blood pressure on the day of surgery and metrics of bleeding.In a preplanned secondary analysis of prospectively collected data from the Limiting IV Chloride to Reduce AKI trial (NCT02020538), univariate and multivariable regression analyses explored the association between peak systolic blood pressure, peak mean arterial pressure, and peak central (...) venous pressure recorded postoperatively on the day of surgery and multiple metrics of bleeding. Patients at increased bleeding risk due to specific criteria were excluded from analysis. The primary outcome was chest tube drainage (milliliters per hour) on the day of surgery. Secondary outcomes included red blood cell transfusion, surgical re-exploration for bleeding, and hospital mortality.The study cohort comprised 793 patients. Mean ± standard deviation peak systolic blood pressure, mean arterial

2019 Journal of Thoracic and Cardiovascular Surgery

169. A simplified blood culture sampling protocol for reducing contamination and costs: a randomized controlled trial. (Abstract)

A simplified blood culture sampling protocol for reducing contamination and costs: a randomized controlled trial. Blood culture contamination carries risks for patients, such as unnecessary antimicrobial therapy and other additional hazards and costs. One method shown to be effective in reducing contamination is initial blood specimen diversion during collection. We hypothesized that initial blood specimen diversion without a designated device or procedure would suffice for reduction in blood (...) culture contamination rate.From 1 September 2017 through to 6 September 2018, we conducted a randomized controlled trial to assess the effect of an initial-specimen diversion technique (ISDT) on the rate of blood-culture contamination by changing the order of sampling using regular vacuum specimen tubes instead of commercially available sterile diversion devices. We included adults from whom the treating physician planned to take blood cultures and additional blood chemistry tests. Additionally, we

2019 Clinical Microbiology and Infection

170. Volume-targeted ventilation with a Fabian ventilator: maintenance of tidal volumes and blood CO<sub>2</sub>. (Abstract)

Volume-targeted ventilation with a Fabian ventilator: maintenance of tidal volumes and blood CO2. To analyse the performance of the Fabian +NCPAP evolution ventilator during volume guarantee (VG) ventilation in neonates at maintaining the target tidal volume and what tidal and minute volumes are required to maintain normocapnia.Clinical and ventilator data were collected and analysed from 83 infants receiving VG ventilation during interhospital transfer. Sedation was used in 26 cases (...) below the target when the endotracheal tube leak exceeded 50%. When leak was below 50%, VTe was below VTset by >1 mL/kg in less than 12% of inflations even in babies weighing less than 1000 g. Both VTe (r=-0.34, p=0.0022) and minute volume (r=-0.22, p=0.0567) showed a weak inverse correlation with capillary partial pressure of carbon dioxide (Pco2) values. Only 50% of normocapnic blood gases were associated with tidal volumes between 4 and 6 mL/kg.The Fabian ventilator delivers volume-targeted

2019 Archives of Disease in Childhood. Fetal and Neonatal Edition

171. Blood monocyte profiles in COPD patients with PiMM and PiZZ α1-antitrypsin. (Abstract)

stable COPD patients with alpha1-antitrypsin (AAT) deficiency (PiZZ, n = 7) and with normal AAT variant (PiMM, n = 7). Peripheral whole blood was collected in sodium heparin tubes and incubated with LPS (from E. coli; 1 μg/ml) or placebo for 6 h at 37 °C, 5% CO2. To profile monocyte subsets we performed flow cytometry analysis based on HLA-DR and CD14/CD16 staining. HLA-DR + subsets of cells did not differ between PiZZ and PiMM COPD, and healthy controls (n = 7), used as a reference. Monocyte (...) Blood monocyte profiles in COPD patients with PiMM and PiZZ α1-antitrypsin. Human blood monocytes are divided into populations based on the differential expression of CD14 and CD16 receptors: CD14 + CD16(classical), CD14 + CD16 + (intermediate), and CD14-CD16+ (non-classical). Given their functional differences and their role in pathogenesis of chronic obstructive pulmonary disease (COPD), monocyte profiling is of clinical interest. Here we investigated blood monocyte subsets in clinically

2019 Respiratory medicine

172. Cheap and rapid in-house method for direct identification of positive blood cultures by MALDI-TOF MS technology. Full Text available with Trip Pro

clinical samples including sterile body fluids. Additional in-house methods enabled direct identification from blood cultures following various preparation protocols.Blood culture (5 ml) was harvested from each positive bottle following growth identification by BACTEC™ FX system and transferred into a VACUETTE® Z Serum Sep Clot Activator tube containing an inert gel, which following centrifugation separates microorganisms from the blood cells. We used MALDI-TOF MS analysis for identification (...) of microorganisms collected from the gel surface.Positive blood culture bottles (186) were collected. In comparison with the routine method, 99% (184/186) and 90% (168/186) of the isolates were correctly identified by the SepsiTyper kit and the in-house method, respectively. We found high concordance (Pearson coefficient = 0.7, p <  0.0001) between our in-house method and the SepsiTyper kit. Additionally, high correlation was found in sub-groups of identified bacteria, with Pearson coefficients of 0.77 (p

2019 BMC Infectious Diseases

173. Conditions for collection of serum samples for the measurement of fibrin(ogen) degradation products by radioimmunoassay of fragment E. Full Text available with Trip Pro

Conditions for collection of serum samples for the measurement of fibrin(ogen) degradation products by radioimmunoassay of fragment E. A number of conditions have been assessed for the collection of serum samples for the measurement of fibrin(ogen) degradation products using a radioimmunoassay for degradation fragment E, which permits precise quantitation of differences. Blood should be collected using minimal venous occlusion into glass tubes containing 10 mg/ml of epsilon amino caproic acid (...) and allowed to clot at 4 to 20 degrees C for at least 4 hours before centrifugation. The serum may be stored at 4 or -20 degrees C. Samples from patients receiving anticoagulant therapy should be treated with 10 IU of thrombin per ml.

1976 Journal of Clinical Pathology

174. Influence of Blood Collection Systems on Coagulation Tests Full Text available with Trip Pro

Influence of Blood Collection Systems on Coagulation Tests Coagulation tests are influenced by pre-analytic conditions such as blood collection systems. Change of glass collection tubes with plastic ones will cause alteration of the test results. The aim of this study was to compare three plastic blood collection tubes with a standard glass blood collection tube and each plastic collection tube with the other two for possible additional tube-to- tube differences.A total of 284 blood samples (...) were obtained from 42 patients receiving warfarin during their routine controls, besides 29 healthy volunteers. Subgroup analyses were done according to health status.Our study demonstrated that different blood collection tubes have a statistically significant influence on coagulation tests. The magnitude of the effect depends on the tube used. However most of the tests performed on samples obtained from any tube correlated significantly with results obtained from other tube samples.Although blood

2012 Turkish Journal of Haematology

175. Blood Collection for Biochemical Analysis in Adult Zebrafish Full Text available with Trip Pro

practitioner. The same technique was used by the same group in another publication in that same year. In 2010, Eames and colleagues assessed whole blood glucose levels in zebrafish. They gained access to the blood by performing decapitations with scissors and then inserting a heparinized microcapillary collection tube into the pectoral articulation. They mention difficulties with hemolysis that were solved with an appropriate storage temperature based on the work Kilpatrick et al. When attempting to use (...) Blood Collection for Biochemical Analysis in Adult Zebrafish The zebrafish has been used as an animal model for studies of several human diseases. It can serve as a powerful preclinical platform for studies of molecular events and therapeutic strategies as well as for evaluating the physiological mechanisms of some pathologies. There are relatively few publications related to adult zebrafish physiology of organs and systems, which may lead researchers to infer that the basic techniques needed

2012 Journal of visualized experiments : JoVE

176. [A comparison of the rates of hemolysis and repeated blood sampling using syringe needles versus vacuum tube needles in the emergency department]. Full Text available with Trip Pro

sampling to determine electrolyte level. ED patients were randomly assigned to either the syringe group or the vacuum tube group. All blood samples were collected by experienced ED nurses and hemolysis was determined by experienced laboratory technologists. Data were analyzed using Fisher's exact test and binary logistic regression.One hundred forty-five valid samples were collected (74 in the syringe group versus 71 in the vacuum tube group). 5 of 74 (6.8%) blood samples in the syringe group and 8 (...) [A comparison of the rates of hemolysis and repeated blood sampling using syringe needles versus vacuum tube needles in the emergency department]. This study was done to compare the rates of hemolysis and repeated sampling in blood samples obtained by a syringe needle versus a vacuum tube needle.A randomized, prospective study was used to evaluate the differences between the two blood sampling methods. The study group consisted of patients seen in the emergency department (ED) for blood

2012 Journal of Korean Academy of Nursing Controlled trial quality: uncertain

177. Diagnostic value of ex vivo pleural fluid interferon-gamma versus adapted whole-blood quantiferon-TB gold in tube assays in tuberculous pleural effusion Full Text available with Trip Pro

Quantiferon-TB Gold In tube assay (QFT- IT) in blood and its adapted variants, using pleural fluid or isolated pleural fluid cells in the diagnosis of pleural TB.Thirty-eight patients with pleural effusion of unknown etiology presented at Assiut University Hospital, Egypt, were recruited. Blood and pleural fluid were collected at presentation for INF-γ assays. Ex vivo pleural fluid INF-γ levels, QFT-IT in blood and its adapted variants were compared with final diagnosis as confirmed by other tools (...) Diagnostic value of ex vivo pleural fluid interferon-gamma versus adapted whole-blood quantiferon-TB gold in tube assays in tuberculous pleural effusion Noninvasive diagnosis of pleural tuberculosis (TB) remains a challenge due to the paucibacillary nature of the disease. As Mycobacterium tuberculosis (MTB)-specific T cells are recruited into pleural space in TB effusion; their indirect detection may provide useful clinical information.Evaluation of pleural fluid interferon (INF)-γ levels vs

2012 Annals Of Thoracic Medicine

178. Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule () containing serum in human microvascular endothelial cell 1. (Abstract)

Effect of EphB4/EphrinB2 reverse signal on angiogenesis induced by Xuefu Zhuyu Capsule () containing serum in human microvascular endothelial cell 1. To evaluate the effect of Xuefu Zhuyu Capsule ()-containing serum (XFZY-CS) on EphB4/ephrinB2 and its reverse signal in human microvascular endothelial cell-1 (HMEC-1).XFZY-CS and the blank control serum were collected. HMEC-1 cells were randomly assigned to 6 groups including the concentration 1.25%, 2.5%, and 5% XFZY-CS groups and their blank (...) serum control ones. The angiogenesis effect of XFZY-CS was tested with an in vitro tube formation assay and the best condition of pro-angiogenesis was determined. The effect of XFZY-CS on EphB4/ephrinB2 and the reverse signal were determined by Western blot and real-time quantitative polymerase chain reaction, respectively; we also confifirmed the results through activating and inhibiting the reverse signal by EphB4/fc and pyrophosphatase/ phosphodiesterase2 (PP2).XFZY-CS promoted angiogenesis

2017 Chinese journal of integrative medicine Controlled trial quality: uncertain

179. Serum Concentration of CEFOxitin Used for Antibiotic Prophylaxis in Obese Patients Undergoing BARiatric Surgery

Date : June 30, 2018 Actual Study Completion Date : August 9, 2018 Resource links provided by the National Library of Medicine related topics: available for: Arms and Interventions Go to Arm Intervention/treatment Bariatric surgery candidate Patients included in this study belong to this arm and put up with intervention "Serum dosage of antibiotic prophylaxis CEFOXITIN" Drug: Serum dosage of antibiotic prophylaxis CEFOXITIN Three blood samples will be collected. These samples are specific (...) for the research, and will be performed on the forearm opposite to the one used for Cefoxitin infusion. Blood sample at the start of surgery (t0): at the end of the Cefoxitin infusion (~30 min), 1 tube (5 ml) of blood will be withdrawn Blood sample at half way surgery (t1): 1 tube (5 ml) of blood will be withdrawn (if applicable) Blood sample at skin closure (t2): 1 tube (5 ml) of blood will be withdrawn Other Name: Drug dosage Outcome Measures Go to Primary Outcome Measures : Serum concentration of Cefoxitin

2017 Clinical Trials

180. Effect of scaling & root planing on the activity of ALP in GCF & serum of patients with gingivitis, chronic and aggressive periodontitis: A comparative study Full Text available with Trip Pro

. Pooled GCF samples collected using micro capillary tubes from the deepest pocket sites for each patient and stored at -70° C. Serum samples also collected and stored at -20° C. Each patient was subjected to scaling and root planing with two weeks maintenance recall. After 6 to 8 weeks GCF and serum samples collected again and all clinical parameters rerecorded. GCF and serum samples analyzed for levels of ALP by using para nitro phenol assay for the three groups.ALP levels in GCF increased (...) Effect of scaling & root planing on the activity of ALP in GCF & serum of patients with gingivitis, chronic and aggressive periodontitis: A comparative study Host response and environmental factors are known to modify periodontal status adversely. Presently serum, saliva, and GCF are being investigated for its biochemical constituents. GCF contains array of biochemical factors, offering potential use as a diagnostic or prognostic biomarker of the biologic state of the periodontium in health

2017 Journal of oral biology and craniofacial research

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