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141. Evaluation in an emergency department of rapid separator tubes containing thrombin for serum preparation prior to hs-cTnT and CK-MB analyses. Full Text available with Trip Pro

Evaluation in an emergency department of rapid separator tubes containing thrombin for serum preparation prior to hs-cTnT and CK-MB analyses. In our emergency department, we collect blood in Rapid Serum Tubes (RSTs; Becton Dickinson, Franklin Lakes, NJ), in which clotting times are reduced. We investigated the influence of RST use on cardiac troponin T (hs-cTnT) and creatine kinase-MB (CK-MB) test results, in comparison with the use of tubes featuring a separator gel containing a clotting (...) activator (SSTs; Green-vac, Yongin, Korea).Samples from 60 patients were divided into equal aliquots and placed into RSTs and SSTs; hs-cTnT and CK-MB concentrations were determined using an autoanalyzer (Elecsys 2010) running commercial assays (Roche Diagnostics, Penzberg, Germany). Between-tube differences in CK-MB and hs-cTnT values were compared using the paired t-test, and correlations among variables were evaluated by calculation of Spearman correlation coefficients (r values). Deming regression

2013 BMC Clinical Pathology

142. Investigation of haptoglobin, serum amyloid A, and some biochemical parameters in calves with omphalitis Full Text available with Trip Pro

were collected from Vena jugularis of animals to tubes with anticoagulant (sodium citrate) and without anticoagulants, pre-operative (day 0), and post-operative (day 7). Samples were used to determine the concentration of haptoglobin (Hp), serum amyloid A (SAA), ceruloplasmin (Cp), fibrinogen, glucose, total protein, albumin, urea, total bilirubin, creatinine, calcium, phosphorus, aspartate aminotransferase (AST), alkaline phosphatase (ALP), and gamma-glutamyl transferase (GGT) concentrations.While (...) Investigation of haptoglobin, serum amyloid A, and some biochemical parameters in calves with omphalitis In this study, it was aimed to determine the concentration of some important acute phase proteins (APPs) and some biochemical parameters pre-operative and post-operative in calves with omphalitis.A total of 20 calves were used in the study and they consist of 10 clinically healthy calves that were used as a control and 10 calves with omphalitis were used as the treatment group. Blood samples

2018 Veterinary world

143. Bias due to Preanalytical Dilution of Rodent Serum for Biochemical Analysis on the Siemens Dimension Xpand Plus Full Text available with Trip Pro

of preanalytical sample dilution on biochemical analysis of mouse and rat serum samples utilizing the Siemens Dimension Xpand Plus. Rats were obtained from end of study research projects. Mice were obtained from sentinel testing programs. For both, whole blood was collected via terminal cardiocentesis into empty tubes and serum was harvested. Biochemical parameters were measured on fresh and thawed frozen samples run straight and at dilution factors 2-10. Dilutions were performed manually, utilizing either (...) Bias due to Preanalytical Dilution of Rodent Serum for Biochemical Analysis on the Siemens Dimension Xpand Plus Clinical pathology testing of rodents is often challenging due to insufficient sample volume. One solution in clinical veterinary and exploratory research environments is dilution of samples prior to analysis. However, published information on the impact of preanalytical sample dilution on rodent biochemical data is incomplete. The objective of this study was to evaluate the effects

2018 Frontiers in veterinary science

144. Serum and Bronchoalveolar Inflammatory Parameters in Patients With Severe Adult Respiratory Distress Syndrome

was to determine if serum MBL levels correlate with the risk of mortality in intensive care units (ICU) patients with A(H1N1)pdm09 infection. Prospective observational study was performed in ICU patients with acute respiratory distress syndrome due to influenza A(H1N1)pdm09 virus. Demographic characteristics and severity indices were recorded at ICU admission. MBL was assayed from blood drawn at influenza diagnosis within 24-48 h following the ICU admission. Outcomes were compared according to MBL levels (...) bacterial infection if positive. 70 control 70 healthy subjects (HS) among blood donors attending the Regional Center for Blood Transfusion (Lille, France). Outcome Measures Go to Primary Outcome Measures : Levels of serum MBL are associated with mortality in critically ill patients with severe A(H1N1)pdm09 viral pneumonia. [ Time Frame: 7days ] The aim of the present study was to determine whether levels of serum MBL are associated with mortality in critically ill patients with severe A(H1N1)pdm09

2018 Clinical Trials

145. The stability and variability of serum and plasma fibroblast growth factor-23 levels in a haemodialysis cohort. Full Text available with Trip Pro

specimen collection methods were examined in a subset of patients (n = 23), with pre-dialysis blood collected into different tubes: plain (serum), EDTA (plasma) and EDTA with the addition of a protease inhibitor (EDTA-PI). All analyses were performed in the main cohort and repeated in each subgroup. Variability over a 3-week period was assessed using repeated measures ANOVA and random effects linear regression models. Intra-class correlation coefficients were calculated to assess agreement (...) ), the intra- and inter-individual variability of FGF-23 levels, and the optimal collection methods remain poorly characterized. In this study we assessed the variability of FGF-23 levels in a cohort of stable haemodialysis patients. Secondly, in a subset of patients, we assessed the effects of different collection methods on measured FGF-23 levels.To assess the variability of FGF-23, pre-dialysis blood samples were collected over 3 consecutive weeks from 75 haemodialysis patients. The effects of different

2018 BMC Nephrology

146. Comparison of Serum Trefoil Factor 3 in Patients With Gingivitis and Periodontitis After Scaling and Root Planing

of blood was collected in each test tube. 10 minutes after collection, the test tube containing 2ml blood was subjected to centrifugation at 3000rpm for 10 min. The supernatant straw colored fluid (serum) was separated into storage vials (Eppendorf tubes) for serum Trefoil Factor 3. Secondary Outcome Measures : Change in the Gingival Index. [ Time Frame: 3 months ] The severity of gingivitis is scored on all surfaces of selected teeth using Williams Periodontal probe. Index teeth- 16, 12, 24, 36, 32 (...) patients whose serum is collected at base line and treated with Scaling and Root Planing and after three months serum is collected for assessment of Trefoil factor 3. Procedure: Scaling and root planing After the serum collection is done from the patient for the assessment of trefoil factor 3, Non surgical periodontal therapy is carried out. Outcome Measures Go to Primary Outcome Measures : Trefoil factor 3 [ Time Frame: 3 months. ] Blood samples were collected by venepuncture of anti-cubital vein. 2ml

2018 Clinical Trials

147. Blood tests: requesting, labelling and sampling requirements

Trust, the current standard equipment is the S-Monovette® System ( ). For blood samples, samples must be drawn/put into bottles in the correct order ( ) GOSH order of draw for the S-Monovette® System Order of draw / Collection sequence Order Tube Additive Common tests 1 Green Sodium citrate Coagulation 2 Brown Serum gel Biochemistry, Immunology, Microbiology and Virology 3 White Serum Biochemistry 4 Orange Lithium heparin Biochemistry, Immunology and Microbiology assay 5 Red EDTA Biochemistry (...) (Not “mother of”). It is NEVER acceptable to use patient forms/stickers with the name changed ( ). Patient identification The completed request form must be taken to the patient and checked against the wristband or other suitable identification (a verbal check of name and DOB may be used for parents/siblings) ( ). Sample collection and labelling All equipment, unlabelled tubes a pen and forms must be taken to the patient’s side. Use of Trust standard equipment is best practice; for Great Ormond Street

2015 Publication 1593

148. Spectra Optia for automatic red blood cell exchange in patients with sickle cell disease

Recommendations Recommendations 1.1 The case for adopting Spectra Optia for automated red blood cell exchange in patients with sickle cell disease is supported by the evidence. Spectra Optia is faster to use and needs to be done less often than manual red blood cell exchange. 1.2 Spectra Optia should be considered for automated red blood cell exchange in patients with sickle cell disease who need regular transfusion. 1.3 NICE recommends collaborative data collection to generate further clinical evidence (...) ://www.nice.org.uk/terms-and- conditions#notice-of-rights). Page 4 of 362 2 The technology The technology Description of the technology 2.1 The Spectra Optia Apheresis System (T erumo BCT) is an apheresis and cell collection platform which can be used in the treatment of sickle cell disease. The purpose of this evaluation is to assess the use of Spectra Optia for automated red blood cell exchange or depletion-exchange in adults or children with sickle cell disease who are on a transfusion regime

2016 National Institute for Health and Clinical Excellence - Medical technologies

149. Determination of anti-HCV and quantification of HCV-RNA and IP-10 from dried blood spots sent by regular mail. Full Text available with Trip Pro

-from dried blood spots, sent by regular mail. We also investigated, if IP-10 determined from dried blood spots correlated with fibrosis progression appraised by transient elastography.Forty chronic HCV infected patients were consecutively enrolled. At baseline and after six months, dried blood spots were prepared from blood collected by venous puncture, dried for 4-6 hours, then stored in gas-impermeable plastic bags with a desiccator, before being sent by regular mail. At each visit, approximately (...) bias of 3 with SD of bias ± 0.6472. We found no correlation between IP-10 and fibrosis progression.We identified anti-HCV in 66/67samples, and quantified IP-10 and HCV-RNA from dried blood spots, dried at room temperature and sent by regular mail. HCV-RNA concentrations from the dried blood spots were lower than corresponding plasma values; a probable result of heparin coated test tubes. We found no correlation between IP-10 and fibrosis progression. Overall, dried blood spots could be a cost

2018 PLoS ONE

150. The effect of blood sampling and preanalytical processing on human N-glycome. Full Text available with Trip Pro

collection, but also influence of various times and temperatures between individual processing steps on the total N-glycome and on a glycan-biomarker score. Venous blood was collected from 10 healthy donors in 11 blood collection tubes with different additives, processed variously to obtain 16 preanalytical variables and N-glycans released from serum or plasma were analyzed by MALDI-TOF-MS and capillary electrophoresis coupled with fluorescence detection (CE-LIF) for the first time. Long time storage (...) of deep frozen samples at -20°C or -80°C exerted only a minor influence on the glycome as demonstrated by CE-LIF. The N-glycome was very stable evidenced by MALDI-TOF when stored at 4°C for at least 48 hours and blood collected in tubes devoid of additives. The glycome was stable upon storage after centrifugation and aliquoting, which is an important information considering future diagnostic applications. Hemolysis, however, negatively correlated with an established glycan score for ovarian cancer

2018 PLoS ONE

151. Hyperacute changes in blood mRNA expression profiles of rats after middle cerebral artery occlusion: Towards a stroke time signature. Full Text available with Trip Pro

samples were collected from 8 male spontaneously hypertensive rats at 0, 1, 2, 3, 6 and 24h post stroke induction by middle cerebral artery occlusion. RNA was extracted from whole blood stabilized in PAXgene tubes and mRNA expression was detected by oligonucleotide Affymetrix microarray. Using a pairwise comparison model, 1932 genes were identified to vary significantly over time (p≤0.5x10-7) within 24h after stroke. Some of the top20 most changed genes are already known to be relevant to the ischemic (...) Hyperacute changes in blood mRNA expression profiles of rats after middle cerebral artery occlusion: Towards a stroke time signature. Stroke evolution is a highly dynamic but variable disease which makes clinical decision making difficult. Biomarker discovery programs intended to aid clinical decision making have however largely ignored the rapidity of stroke evolution. We have used gene array technology to determine blood mRNA expression changes over the first day after stroke in rats. Blood

2018 PLoS ONE

152. Quality Assessment of Platelet-Rich Fibrin-Like Matrix Prepared from Whole Blood Samples after Extended Storage Full Text available with Trip Pro

extended the storage time of WB samples from 2 to 7 days and assessed the quality of the resulting PRFMs. WB samples collected with acid-citrate-dextrose were stored with gentle agitation at ambient temperature. To prepare PRFMs, the stored WB samples were mixed with CaCl₂ in glass tubes and centrifuged. Fibrin fiber networks, CD41 and CD62P expression, and Platelet Derived Growth Factor-BB (PDGF-BB) levels were examined by scanning electron microscopy (SEM), flow cytometry, and an Enzyme-Linked (...) Quality Assessment of Platelet-Rich Fibrin-Like Matrix Prepared from Whole Blood Samples after Extended Storage The platelet-rich fibrin-like matrix (PRFM) is usually prepared onsite and immediately used for regenerative therapy. Nonetheless, to meet the clinical necessity of preserving the PRFM without quality deterioration, we developed a method for preparation of PRFMs from short-term-stored whole blood (WB) samples. In this study, to evaluate the practical expiration date of storage, we

2017 Biomedicines

153. Increased IRS2 mRNA Expression in SGA Neonates: PCR Analysis of Insulin/IGF Signaling in Cord Blood Full Text available with Trip Pro

Center of Tottori University Hospital.Fifty-two [42 appropriate-for-gestational-age (AGA) and 10 SGA] neonates.Immediately collected cord blood was placed into a PAXgene Blood RNA Tube. Total RNA from the blood was purified using reagents provided in the PAXgene Blood RNA Kit within 4 days, and reverse transcription polymerase chain reaction (PCR) was performed.Quantitative real-time PCR analysis was applied to evaluate the mRNA expression of insulin receptor (INSR), IGF-I receptor (IGF1R), insulin (...) Increased IRS2 mRNA Expression in SGA Neonates: PCR Analysis of Insulin/IGF Signaling in Cord Blood Hypoglycemia is the most common metabolic problem among small-for-gestational-age (SGA) neonates. However, the pathological mechanism and insulin/ insulin-like growth factor (IGF) signaling axis in neonates remain unknown.To determine the insulin/IGF axis in neonates, we analyzed the messenger RNA (mRNA) expression of insulin/IGF signaling in fetal umbilical cord blood.The Perinatal Medical

2017 Journal of the Endocrine Society

154. Quality Impact on Diagnostic Blood Specimen Collection Using a New Device to Relieve Venipuncture Pain Full Text available with Trip Pro

was located on the left forearm without applying tourniquet, in order to prevent any interference from venous stasis, and blood samples were collected using a 20-G straight needle directly into 5 mL vacuum tubes with clot activator and gel separator. In sequence, external cold and vibration by Buzzy(®) was applied on the right forearm-5 cm above the chosen puncture site-for 1 min before venipuncture and continued until the end of the same procedure already done in the left forearm. The panel of tests (...) Quality Impact on Diagnostic Blood Specimen Collection Using a New Device to Relieve Venipuncture Pain A new device called Buzzy(®) has been recently presented that combines a cooling ice pack and a vibrating motor in order to relieve the venipuncture pain. The aim of this study was to evaluate the impact of Buzzy(®) use during diagnostic blood specimen collection by venipuncture for routine immunochemistry tests. Blood was collected from 100 volunteers by a single, expert phlebotomist. A vein

2013 Indian Journal of Clinical Biochemistry

155. Critical review and meta-analysis of spurious hemolysis in blood samples collected from intravenous catheters. Full Text available with Trip Pro

and Scopus to estimate the risk of spurious hemolysis in blood samples collected from intravenous catheters. A meta-analysis with calculation of Odds ratio (OR) and Relative risk (RR) along with 95% Confidence interval (95% CI) was carried out using random effect mode.Fifteen articles including 17 studies were finally selected. The total number of patients was 14,796 in 13 studies assessing catheter and evacuated tubes versus straight needle and evacuated tubes, and 1251 in 4 studies assessing catheter (...) and evacuated tubes versus catheter and manual aspiration. A significant risk of hemolysis was found in studies assessing catheter and evacuated tubes versus straight needle and evacuated tubes (random effect OR 3.4; 95% CI = 2.9-3.9 and random effect RR 1.07; 95% CI = 1.06-1.08), as well as in studies assessing catheter and evacuated tubes versus catheter and manual aspiration of blood (OR 3.7; 95% CI = 2.7-5.1 and RR 1.32; 95% CI = 1.24-1.40).Sample collection through intravenous catheters is associated

2013 Biochemia medica

156. A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation Full Text available with Trip Pro

A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation Laboratory tests can be done on the cellular or fluid portions of the blood. The use of different blood collection tubes determines the portion of the blood that can be analyzed (whole blood, plasma or serum). Laboratories involved in studying the genetic basis of human disorders rely on anticoagulated whole blood collected in EDTA-containing vacutainer as the source of DNA for genetic (...) and DNA, that method would be considered an advancement to existing methods. The use of the compacted blood after plasma extraction represents an alternative source for genomic DNA, thus minimizing the amount of blood samples processed and reducing the number of samples required from each patient. This would ultimately save time and resources. The BD P100 blood collection system for plasma protein preservation were created as an improved method over previous plasma or serum collection tubes(1

2013 Journal of visualized experiments : JoVE

157. Prospective Collection of Surplus Surgical Tumor Tissues and Pre-surgical Blood Samples

diagnosis and will never interfere with clinical diagnosis. Blood Samples Blood samples will be collected from consented subjects prior to surgery and will involve the collection of up to 20 ml of whole blood into appropriate blood collection tubes and freezing them. Eligibility Criteria Go to Information from the National Library of Medicine Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn (...) Prospective Collection of Surplus Surgical Tumor Tissues and Pre-surgical Blood Samples Prospective Collection of Surplus Surgical Tumor Tissues and Pre-surgical Blood Samples - Full Text View - ClinicalTrials.gov Hide glossary Glossary Study record managers: refer to the if submitting registration or results information. Search for terms x × Study Record Detail Saved Studies Save this study Warning You have reached the maximum number of saved studies (100). Please remove one or more studies

2013 Clinical Trials

158. Translational Research in Pediatrics II: Blood Collection, Processing, Shipping, and Storage. Full Text available with Trip Pro

Translational Research in Pediatrics II: Blood Collection, Processing, Shipping, and Storage. Translational research often involves tissue sampling and analysis. Blood is by far the most common tissue collected. Due to the many difficulties encountered with blood procurement from children, it is imperative to maximize the quality and stability of the collected samples to optimize research results. Collected blood can remain whole or be fractionated into serum, plasma, or cell concentrates (...) researchers working with human blood are aware of how experimental results can be altered by blood sampling methods, times to processing, container tubes, presence or absence of additives, shipping and storage variables, and freeze-thaw cycles. The authors of this review, in an effort to encourage and optimize translational research using blood from pediatric patients, outline best practices for blood collection, processing, shipment, and storage.

2013 Pediatrics

159. Prevention of hemolysis in blood samples collected from intravenous catheters. (Abstract)

Prevention of hemolysis in blood samples collected from intravenous catheters. Samples drawn through intravenous catheters are frequently hemolyzed. We planned a prospective, randomized study to establish whether hemolysis in samples drawn from intravenous catheters may be reduced using S-Monovette® tubes collected by manual aspiration as compared with standard vacuum tubes.We studied 52 consecutive patients admitted to the ED. Blood was drawn through a 20-gauge intravenous catheter. A 5.0mL (...) , Becton Dickinson Vacutainer® SST II Plus serum tube was collected and discarded. In the odd group of patients (i.e., n. 1, 3, 5, etc.), a second SST II tube was drawn with vacuum ("BD-V"), followed by a 5.5mL S-Monovette® serum tube collected with manual aspiration ("SD-A") and an identical S-Monovette collected by vacuum ("SD-V"). In the pair group of patients (i.e., n. 2, 4, 6, etc.), the sequence was modified to "SD-A", "SD-V" and "BD-V". Serum was separated and tested for lactate dehydrogenase

2013 Clinical biochemistry Controlled trial quality: uncertain

160. Impact of a large-scale educational intervention program on venous blood specimen collection practices. Full Text available with Trip Pro

Impact of a large-scale educational intervention program on venous blood specimen collection practices. Phlebotomy performed with poor adherence to venous blood specimen collection (VBSC) guidelines jeopardizes patient safety and may lead to patient suffering and adverse events. A first questionnaire study demonstrated low compliance to VBSC guidelines, motivating an educational intervention of all phlebotomists within a county council. The aim was to evaluate the impact of a large-scale (...) control, release of venous stasis, and test tube labelling had significantly improved in the intervention group but did not significantly differ from the control group (ES = 0.22- 0.49, p = < 0.001- 0.006). The control group showed no significant improvements at all (ES = 0-0.39, p = 0.016-0.961).The present study demonstrated several significant improvements on phlebotomists' adherence to VBSC practices. Still, guideline adherence improvement to several crucial phlebotomy practices is needed. We

2013 BMC Health Services Research

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