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61. Effect of the size of evacuated blood collection tubes on total carbon dioxide concentration in equine plasma. (PubMed)

Effect of the size of evacuated blood collection tubes on total carbon dioxide concentration in equine plasma. To determine whether plasma total CO(2) concentrations would vary with the size of the evacuated tube used to collect blood samples.Randomized crossover study.Convenience sample of 20 healthy adult horses.Jugular venous blood was collected from horses in random order into 8 types of evacuated tubes: 2-mL glass, 2- or 3-mL plastic or plastic plasma separator, 4- or 6-mL plastic, and 10 (...) -mL glass or plastic. Total CO(2) concentrations in plasma were measured with a biochemistry analyzer. Data were analyzed via repeated-measures ANOVA and multivariate regression.The air volume-to-blood volume ratio was significantly higher and consequently, plasma total CO(2) concentration was significantly lower when blood was collected into 2-mL glass tubes and 2- or 3-mL plastic tubes than when the other 5 types of evacuated tubes were used. Concentrations in the other tube types were

2012 Journal of the American Veterinary Medical Association Controlled trial quality: uncertain

62. Prenatal Screening for Down Syndrome, Trisomy 18, and Open Neural Tube Defect

trimester ultrasound 2 are some of the options available for prenatal genetic screening? The risks for fetal Down syndrome, trisomy 18, and open neural tube defects (ONTDs) are calculated using a combination of variables which may include: biochemical serum markers collected from blood work, maternal age, maternal ethnicity, maternal weight, maternal diabetic status, maternal smoking, and, if available, nuchal translucency (NT) ultrasound measurement? There are four different screening tests: SIPS, IPS (...) A (PAPP-A) and second trimester quad markers in two separate blood tests? Quad markers include alpha-fetoprotein (AFP), unconjugated estriol (uE3), human chorionic gonadotropin (hCG) and inhibin-A? The first blood test is collected between 9 – 13 +6 weeks (best at 10 – 11 +6 weeks) and the second between 14 – 20 +6 weeks (best at 15 – 16 weeks)? Test results are available within 10 days after the second blood test? Both blood tests can be collected in the woman’s local community with samples being

2014 British Columbia Perinatal Health Program

63. Nephrostomy and Biliary Tube Management

in the context of infection. An absence of urine, presence of blood in the collection bag, or flank pain may indicate that the tube is blocked. 24 If blockage is suspected it was recommended that nephrostomy tubes be irrigated or flushed using gentle force with normal saline or sterile water using sterile or aseptic technique. 4,6,9,10,24 Two documents stated that hand washing before and after flushing the nephrostomy tube is essential and sterile gloves should be worn. 6,24 Four guidelines recommend (...) Nephrostomy and Biliary Tube Management TITLE: Nephrostomy and Biliary Tube Management: A Review of the Clinical Evidence and Guidelines DATE: 4 September 2014 CONTEXT AND POLICY ISSUES Nephrostomy and biliary tubes are drainage catheters that are placed into the renal collecting system or biliary tree and assist in draining urine or bile, respectively. The most common indication for nephrostomy or biliary tubes is obstruction of the renal collecting system or biliary tree. 1-6 Obstruction can

2014 Canadian Agency for Drugs and Technologies in Health - Rapid Review

64. Diagnostic accuracy of ESAT-6 free IGRA compared to QuantiFERON-TB Gold In-tube. (PubMed)

in comparison to QuantiFERON-TB Gold In-Tube (QFT).Participants with different levels of M.tb exposure and TB disease were enrolled to determine the ESAT-6 free IGRA cut-off, to test assay performance in independent cohorts compared to standard QFT and to perform a technical qualification of antigen-coated blood collection tubes.ESAT-6 free IGRA antigen recognition was evaluated in South African adolescents with positive and negative QFT status. The ESAT-6 free IGRA cut-off was established at 0.61 IU/mL (...) , based on ROC analysis in M.tb-unexposed controls and microbiologically-confirmed pulmonary TB patients. In an independent cohort of healthy South African adolescents, levels of IFN released in QFT and ESAT-6 free IGRA were highly correlated (p<0.0001, r=0.83) and yielded comparable positivity rates: 41.5% and 43.5% respectively, with 91% concordance between the tests (kappa = 0.82, 95% CI: 0.74-0.90; McNemar's p=0.48). ESAT-6 free IGRA blood collection tubes had acceptable lot-to-lot variability

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2019 Clinical Infectious Diseases

65. Pharmacokinetic Profile of Prolonged-Release Tacrolimus When Administered via Nasogastric Tube in De Novo Liver Transplantation: A Sub-Study of the DIAMOND Trial. (PubMed)

novo liver transplant recipients, we investigated the pharmacokinetic (PK) profile of prolonged-release tacrolimus when administered via nasogastric tube immediately post-transplant. PK analyses were performed on whole-blood samples collected on Day 1 of tacrolimus administration and on Day 3 post-transplantation. Endpoints included AUC0-24, Cmax, Tmax, and Cmin. RESULTS In total, 10 patients were included in the PK sub-study. The overall mean daily dose of prolonged-release tacrolimus administered (...) Pharmacokinetic Profile of Prolonged-Release Tacrolimus When Administered via Nasogastric Tube in De Novo Liver Transplantation: A Sub-Study of the DIAMOND Trial. BACKGROUND For patients unable to swallow during the immediate post-transplant period, immunosuppressant therapy may be initiated by administering prolonged-release tacrolimus as a suspension via a nasogastric tube. MATERIAL AND METHODS In this sub-study of the DIAMOND randomized controlled trial of prolonged-release tacrolimus in de

2019 Annals of transplantation Controlled trial quality: uncertain

66. Measurement of Blood Pressure in Humans

Measurement of Blood Pressure in Humans Measurement of Blood Pressure in Humans: A Scientific Statement From the American Heart Association | Hypertension Search Hello Guest! Login to your account Email Password Keep me logged in Search May 2019 April 2019 March 2019 February 2019 January 2019 This site uses cookies. By continuing to browse this site you are agreeing to our use of cookies. Free Access article Share on Jump to Free Access article Measurement of Blood Pressure in Humans (...) May 2019 Hypertension. 2019;73:e35–e66 Abstract The accurate measurement of blood pressure (BP) is essential for the diagnosis and management of hypertension. This article provides an updated American Heart Association scientific statement on BP measurement in humans. In the office setting, many oscillometric devices have been validated that allow accurate BP measurement while reducing human errors associated with the auscultatory approach. Fully automated oscillometric devices capable of taking

2019 American Heart Association

67. Specimen collection - microbiology and virology

three separate collection tubes of CSF when investigating for evidence of subarachnoid haemorrhage, as the initial part of the sample may be contaminated with blood from outside the sub-arachnoid space. If this is performed, it is important to label the tubes as such and specifically request counts on the first and third samples. It is also important to remember that a CSF glucose level (sent separately to chemical pathology) can only be accurately interpreted in conjunction with a simultaneous (...) , central venous catheter) When taking samples from intact skin the swab should be moistened with sterile 0.9% saline solution before sampling as this assists in the transfer of bacteria from the sampling site to swab and can increase the number of microorganisms collected ( ). Requests have to be discussed with a Consultant Microbiologist or Clinical Scientist if uncertain of the suitability of a specimen or the availability of a test. Specimen collection: Blood should be sent in an EDTA tube. All

2014 Publication 1593

68. Use of QuantiFERON®-TB Gold in-tube culture supernatants for measurement of antibody responses. (PubMed)

Use of QuantiFERON®-TB Gold in-tube culture supernatants for measurement of antibody responses. QuantiFERON®-TB Gold in-tube (QFT-GIT) supernatants may be important samples for use in assessment of anti-tuberculosis (TB) antibodies when only limited volumes of blood can be collected and when a combination of antibody and cytokine measurements are required. These analytes, when used together, may also have the potential to differentiate active pulmonary TB (APTB) from latent TB infection (LTBI (...) % and specificities of 41.4% & 51.7% respectively. Anti-CFP-10 antibody/M.tb specific IFN-γ and anti-ESAT-6 antibody/M.tb specific IFN-γ ratios had sensitivities of 48.5% & 54.6% and specificities of 89.7% and 75.9% respectively. We conclude that QFT-GIT nil supernatants may be used in the place of sera when measuring antibody responses, reducing blood volumes needed for such investigations. Antibodies in QFT-GIT nil supernatants on their own discriminate APTB from LTBI with high sensitivity but have poor

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2017 PLoS ONE

69. Niraparib (Zejula) - Fallopian Tube Neoplasms, Ovarian Neoplasms, Peritoneal Neoplasms

Niraparib (Zejula) - Fallopian Tube Neoplasms, Ovarian Neoplasms, Peritoneal Neoplasms 30 Churchill Place ? Canary Wharf ? London E14 5EU ? United Kingdom An agency of the European Union Telephone +44 (0)20 3660 6000 Facsimile +44 (0)20 3660 5520 Send a question via our website www.ema.europa.eu/contact 14 September 2017 EMA/648982/2017 Committee for Medicinal Products for Human Use (CHMP) Assessment report Zejula International non-proprietary name: niraparib Procedure No. EMEA/H/C/004249/0000 (...) reactions AE adverse event AESI adverse event of special interest ALT alanine aminotransferase AME absorption, metabolism, excretion AML acute myeloid leukemia ANC absolute neutrophil count ASCN allele specific copy number AST aspartate aminotransferase ATM ataxia telangiectasia mutated AUC area under the plasma concentration-time curve AUCtau area under the plasma concentration-time curve over the dosing interval BBB blood brain barrier BCS Biopharmaceutics Classification System BER base excision

2017 European Medicines Agency - EPARs

70. RNase H2-Dependent Polymerase Chain Reaction and Elimination of Confounders in Sample Collection, Storage, and Analysis Strengthen Evidence That microRNAs in Bovine Milk Are Bioavailable in Humans. (PubMed)

participants before and 6 h after consumption of 1.0 L of 1%-fat bovine milk.The use of heparin tubes for blood collection resulted in a complete loss of miRs. Circadian variations did not affect the concentrations of 8 select miRs. Erythrocyte hemolysis caused artifacts for some miRs if plasma absorbance at 414 nm was >0.300. The stability of plasma miRs depended greatly on the matrix in which the miRs were stored and whether the plasma was frozen before analysis. Purified miR-16, miR-200c, and cel-miR-39 (...) were to assess potential confounders of plasma miR analysis and to detect miRs from bovine milk in human plasma.Potential confounders of plasma miR analysis (circadian rhythm, sample collection and storage, calibration, and erythrocyte hemolysis) were assessed by quantitative reverse transcriptase polymerase chain reaction (PCR) by using blood from healthy adults (7 men, 6 women; aged 23-57 y). Bovine miRs were analyzed by RNase H2-dependent PCR (rhPCR) in plasma collected from a subcohort of 11

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2018 Journal of Nutrition

71. Sample collection and sample handling errors submitted to the transfusion error surveillance system, 2006 to 2015. (PubMed)

information regarding institutional policies. Samples received in the blood bank were used to calculate rates. "Wrong blood in tube" (WBIT) errors are blood taken from wrong patient and labeled with intended patient's information, or blood taken from intended patient but labeled with another patient's information.A total of 42,363 SC and 14,666 SH errors were reported. Predefined low-severity (low potential for harm) and high-severity errors (potential for fatal outcomes) increased from 2006 to 2015 (low (...) Sample collection and sample handling errors submitted to the transfusion error surveillance system, 2006 to 2015. In Canada, transfusion-related errors are voluntarily reported to a tracking system with the goal to systematically improve transfusion safety. This report provides an analysis of sample collection (SC) and sample handling (SH) errors from this national error-tracking system.Errors from 2006 to 2015 from 23 participating sites were extracted. A survey was conducted to obtain

2018 Transfusion

72. The Chinese Children and Families Cohort Study: The Nutrition, Physical Activity, and Ultraviolet Radiation Data Collection (PubMed)

The Chinese Children and Families Cohort Study: The Nutrition, Physical Activity, and Ultraviolet Radiation Data Collection This article reports the study design, methodological issues and early results of a pilot study testing methods for collecting nutrition, physical activity, and ultraviolet (UV) radiation exposure data in a groundbreaking study in China. Epidemiological studies suggest that exposures across the entire life course, including in utero, early childhood, and adolescence, may (...) be important in the etiology of adult cancers and other chronic diseases. The Chinese Children and Families Cohort Study intends to follow-up subjects from the 1993 to 1995 Community Intervention Program of folic acid supplementation for the prevention of neural tube defects. This cohort is unique in that only folic acid exposure during pregnancy varies between groups as other supplements were not available, and there were nutrient deficiencies in the populations. Prior to launching a large-scale follow-up

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2018 Nutrition today

73. Sample Collection From Healthy Volunteers for Assay Optimization

, participants will be asked to give one or more of these samples: Blood will be drawn from an arm vein with a needle and syringe. Mucus and skin will be collected by rubbing the area with a cotton swab. The areas may include the top of the tongue, inside the cheek, nostrils, behind the ear, elbow pit, or vagina. Participants will spit into a tube to collect saliva. Participants will pass stool into a plastic container that fits in the toilet under the seat. They will get sampling kits and instructions. Over (...) samples like blood and cells. In order to do its work, LISB needs to have a lot of these samples available. Objective: To collect biological specimens to use for designing and improving research tests. Specimens include blood, stool, saliva, and skin/mucosal swabs. Eligibility: Healthy people ages 18-80 Design: Participants will be screened with an interview about their general health and their medical history. They will have a physical exam and blood tests. If the results of the screening are normal

2018 Clinical Trials

74. Effects of Collection and Processing Procedures on Plasma Circulating Cell-Free DNA from Cancer Patients (PubMed)

of the delay in processing, storage temperatures, different blood collection tubes, centrifugation protocols, and sample shipment on cfDNA levels. Peripheral blood (n = 231) from cancer patients (n = 62) were collected into K3EDTA or Cell-free DNA BCT tubes and analyzed by digital PCR, targeted amplicon, or shallow whole-genome sequencing. To assess pre-analytic effects, plasma was processed under different conditions after 0, 6, 24, 48, 96 hours, and 1 week at room temperature or 4°C, or using different (...) Effects of Collection and Processing Procedures on Plasma Circulating Cell-Free DNA from Cancer Patients Circulating tumor DNA (ctDNA) offers new opportunities for noninvasive cancer management. Detecting ctDNA in plasma is challenging because it constitutes only a minor fraction of the total cell-free DNA (cfDNA). Pre-analytical factors affect cfDNA levels contributed from leukocyte lysis, hence the ability to detect low-frequency mutant alleles. This study investigates the effects

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2018 The Journal of molecular diagnostics : JMD

75. Evacuated blood-collection tubes--the backflow hazard. (PubMed)

Evacuated blood-collection tubes--the backflow hazard. Five cases of nosocomial infection caused by Serratia marcescens were traced to backflow of blood from nonsterile evacuated blood-collection tubes. The mechanism of backflow was investigated theoretically and the conditions were determined under which backflow can occur. The theory was confirmed by experiments conducted on a simulated venous system and by measurements of the venous pressure in the brachial vein of a patient during (...) application and removal of a tourniquet. Various possible solutions to the backflow hazard include strict adherence to correct venipuncture technique, sterilization of all blood-collection tubes, improvement of the vacuum in the tubes and incorporation of a check valve into the system.

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1975 Canadian Medical Association Journal

76. Blood specimen collection tubes for coagulation tests. (PubMed)

Blood specimen collection tubes for coagulation tests. 500841 1980 01 19 2018 11 13 0021-9746 32 7 1979 Jul Journal of clinical pathology J. Clin. Pathol. Blood specimen collection tubes for coagulation tests. 741 Blackburn E K EK Shinton N K NK eng Letter England J Clin Pathol 0376601 0021-9746 0 Plastics AIM IM Blood Coagulation Tests instrumentation Blood Specimen Collection instrumentation Plastics 1979 7 1 1979 7 1 0 1 1979 7 1 0 0 ppublish 500841 PMC1145789 Thromb Haemost. 1976 Aug 31;36

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1979 Journal of Clinical Pathology

77. Blood specimen collection tubes for coagulation tests. (PubMed)

Blood specimen collection tubes for coagulation tests. 435872 1979 07 16 2018 11 13 0007-1447 1 6167 1979 Mar 31 British medical journal Br Med J Blood specimen collection tubes for coagulation tests. 894-5 Blackburn E K EK Shinton N K NK eng Letter England Br Med J 0372673 0007-1447 AIM IM Blood Coagulation Tests Blood Specimen Collection instrumentation 1979 3 31 1979 3 31 0 1 1979 3 31 0 0 ppublish 435872 PMC1598518 Thromb Haemost. 1976 Aug 31;36(1):237-8 1036814

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1979 British medical journal

78. Use of Polyethylene Tubing for Collecting Small Blood Samples (PubMed)

Use of Polyethylene Tubing for Collecting Small Blood Samples 13240572 2003 05 01 2018 12 01 0008-4409 73 2 1955 Jul 15 Canadian Medical Association journal Can Med Assoc J Use of polyethylene tubing for collecting small blood samples. 135 OGILVIE G F GF PETT L B LB eng Journal Article Canada Can Med Assoc J 0414110 0008-4409 0 Polyethylenes 9002-88-4 Polyethylene OM Blood Blood Specimen Collection Humans Polyethylene Polyethylenes 5528:52215:87:453 BLOOD POLYETHYLENE 1955 7 15 1955 7 15 0 1

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1955 Canadian Medical Association Journal

79. Association of Anaesthetists guidelines: cell salvage for peri-operative blood conservation 2018

objection to receiving an allogeneic transfusion. If there is doubt about expected blood loss, then we recommend that the most cost effective measure is to set up only the suction and anticoagulation tubing and reservoir of the CS device (so‐called ‘collect only’). The blood processing system is then set up during surgery only if enough blood has been collected for processing to be worthwhile, usually more than 500 ml . Cell salvage is commonly used during the following types of surgery: cardiac; major (...) that reduces the requirement for and amount of allogeneic blood transfusion and maintains postoperative haemoglobin concentration. Cell salvage is a method of autologous blood transfusion. It involves the collection of shed blood during and immediately after surgery, which is re‐infused back to the same patient. Intra‐operative CS usually involves the use of a device which processes shed blood; however, postoperatively, unwashed filtered blood can be re‐infused under certain circumstances. Currently, NICE

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2018 Association of Anaesthetists of GB and Ireland

80. Jehovah's Witnesses and patients who refuse blood

normovolaemic haemodilution [23]; blood is siphoned off by gravity into a collection bag containing anticoagulant, and replaced with crystalloid or colloid solution; if crystalloid is used it is likely that a larger volume will need to be administered than the volume of blood withdrawn in order to maintain normovolaemia. Surgicalbloodloss,therefore,hasalowerhaematocritand theblood removedfrom thepatient is re-infused during or attheendofsurgery.Localpolicyandguidelinesshouldbe (...) considered according to the individual clinical situation [29]. The volume of blood samples should be minimised by using paediatric sampling tubes if possible, and point-of-care testingshouldbeavailable. Following surgical procedures that employed a tourniquet intra-operatively there may be excessive blood lossafteritsreleasethatmaycontinueintothepostoperative period. In these situations, postoperative CS can be employed. In case of bleeding, a postoperative dose of intravenoustranexamicacid(1 g

2018 Association of Anaesthetists of GB and Ireland

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