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41. Virtual Global Transplant Laboratory Standard Operating Procedures for Blood Collection, PBMC Isolation, and Storage (PubMed)

that maximize survival and functionality of PBMC at the time of analysis. This resource includes detailed protocols describing blood collection tubes, isolation of PBMC using a density gradient, cryopreservation of PBMC, and thawing of cells as well as preparation for functional assays. For each protocol, we include important considerations, such as timing, storage temperatures, and freezing rate. In addition, we provide alternatives so that researchers can make informed decisions in determining the optimal (...) Virtual Global Transplant Laboratory Standard Operating Procedures for Blood Collection, PBMC Isolation, and Storage Research on human immune responses frequently involves the use of peripheral blood mononuclear cells (PBMC) immediately, or at significantly delayed timepoints, after collection. This requires PBMC isolation from whole blood and cryopreservation for some applications. It is important to standardize protocols for blood collection, PBMC isolation, cryopreservation, and thawing

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2016 Transplantation direct

42. Comparison of the results of two chest tube managements during an enhanced recovery program after video-assisted thoracoscopic lobectomy: A randomized trial. (PubMed)

open label randomized clinical trial and equally divided into two groups. The data collected in the trial included age, gender, forced expiratory volume in 1 second (FEV1), blood loss, operation time, drainage volume, drainage time, length of stay, postoperative pain score according to the Visual Analogue Scale (VAS) within 24 hours after surgery and chest tube removal. This study was registered at ClinicalTrials.gov (NCT03598296).The characteristics of the patients were similar in both groups (...) Comparison of the results of two chest tube managements during an enhanced recovery program after video-assisted thoracoscopic lobectomy: A randomized trial. This study compared the results of the application of two different chest tube management systems; a drainage ball with low negative pressure and the more commonly used chest tube with water-sealed bottle, after video-assisted thoracoscopic (VATS) lobectomy.A total of 60 patients undergoing lobectomy were enrolled into this prospective

2019 Thoracic cancer Controlled trial quality: uncertain

43. Comparison of blood RNA isolation methods from samples stabilized in Tempus tubes and stored at a large human biobank (PubMed)

Comparison of blood RNA isolation methods from samples stabilized in Tempus tubes and stored at a large human biobank More than 50,000 adult and cord blood samples were collected in Tempus tubes and stored at the Norwegian Institute of Public Health Biobank for future use. In this study, we systematically evaluated and compared five blood-RNA isolation protocols: three blood-RNA isolation protocols optimized for simultaneous isolation of all blood-RNA species (MagMAX RNA Isolation Kit, both (...) manual and semi-automated protocols; and Norgen Preserved Blood RNA kit I); and two protocols optimized for large RNAs only (Tempus Spin RNA, and Tempus 6-port isolation kit). We estimated the following parameters: RNA quality, RNA yield, processing time, cost per sample, and RNA transcript stability of six selected mRNAs and 13 miRNAs using real-time qPCR.Whole blood samples from adults (n = 59 tubes) and umbilical cord blood (n = 18 tubes) samples collected in Tempus tubes were analyzed. High

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2016 BMC research notes

44. Red Blood Cell Folate Insufficiency among Nonpregnant Women of Childbearing Age in Guatemala 2009 to 2010: Prevalence and Predicted Neural Tube Defects Risk (PubMed)

Red Blood Cell Folate Insufficiency among Nonpregnant Women of Childbearing Age in Guatemala 2009 to 2010: Prevalence and Predicted Neural Tube Defects Risk The World Health Organization recently released recommendations stating that red blood cell (RBC) folate concentrations should be above 400 ng/L (906 nmol/L) for optimal prevention of folate-sensitive neural tube defects (NTDs). The objective of this study was to determine the distribution of folate insufficiency (FI) (<906 nmol/L (...) ) and potential risk of NTDs based on RBC folate concentrations among nonpregnant women of child-bearing age in Guatemala.A national and regional multistage cluster probability survey was completed during 2009 to 2010 among Guatemalan women of child-bearing age 15 to 49 years of age. Demographic and health information and blood samples for RBC folate analyses were collected from 1473 women. Prevalence rate ratios of FI and predicted NTD prevalence were estimated based on RBC folate concentrations comparing

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2016 Birth defects research. Part A, Clinical and molecular teratology

45. Folic acid fortified milk increases blood folate to concentrations associated with a very low risk of neural tube defects in Singaporean women of childbearing age. (PubMed)

Folic acid fortified milk increases blood folate to concentrations associated with a very low risk of neural tube defects in Singaporean women of childbearing age. Folic acid (400 μg/d) taken during the periconceptional period reduces neural tube defect (NTD) risk by >75%. Achieving red cell folate (RCF) or plasma folate (PF) >905 nmol/L and >35 nmol/L, respectively, has been associated with a low risk of NTDs. We determined whether daily consumption of folic acid fortified milk increases blood (...) folate concentrations to levels associated with a low risk of NTDs in Singaporean women of childbearing age.In this double-blind placebo-controlled trial, 70 non-pregnant women (21-35 y) were randomly assigned to receive fortified milk (FM) powder providing 400 μg folic acid per day or unfortified placebo milk (PM) powder for 12 weeks. Blood samples were collected at baseline and at 6 and 12 weeks.At 12 weeks, mean (95% CI) RCF and PF concentrations were 376 (240, 512) and 39 (26, 51) nmol/L higher

2016 Asia Pacific journal of clinical nutrition Controlled trial quality: predicted high

46. Accuracy of laboratory tests collected at referring hospitals versus tertiary care hospitals for acute stroke patients. (PubMed)

these blood samples are valid for clinical use and the present study aimed to confirm or deny their validity.A diagnostic study was conducted from June 2015 to May 2016. After exclusion, 340 patients were deemed eligible for analysis. Blood samples were collected just before normal saline infusion at referring hospitals and stored in blood collecting tube boxes set during transportation. At the stroke center, informed consents was requested, blood samples were re-collected to serve as a 'gold standard (...) Accuracy of laboratory tests collected at referring hospitals versus tertiary care hospitals for acute stroke patients. The standard treatment of acute ischemic stroke patients is thrombolytic therapy within 60 minutes of a patient's arrival in stroke center hospitals. Based on the policy of the Lampang Referral System Committee, blood samples of suspected stroke patients need to be collected before transfer to the stroke center (Lampang Hospital). It was still questionable as to whether

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2019 PLoS ONE

47. The local clinical validation of a new lithium heparin tube with a barrier: BD Vacutainer® Barricor LH Plasma tube (PubMed)

The local clinical validation of a new lithium heparin tube with a barrier: BD Vacutainer® Barricor LH Plasma tube Although serum-providing blood tubes with a barrier are still widely used due to their significant advantages, the use of blood tubes with a barrier to provide plasma is becoming widespread. We compared 22 analytes in a BD Vacutainer® Barricor LH Plasma tube for local clinical validation of this new lithium heparin tube with a barrier.Samples from 44 volunteers were collected (...) in different tubes (Becton Dickinson and Company): Z tube without additive (reference), clot-activator tube with gel (SST), lithium heparin tube without gel (LiH), and lithium heparin tube with barrier (Barricor). Analyte concentrations in different tubes were compared with the reference tube. All tubes were also evaluated according to additional testing (different centrifugation durations, blood-sampling techniques and individual differences).Aspartate aminotransferase (AST), glucose (Glc), potassium (K

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2017 Biochemia medica

48. Chemically Modified Plastic Tube for High Volume Removal and Collection of Circulating Tumor Cells (PubMed)

Chemically Modified Plastic Tube for High Volume Removal and Collection of Circulating Tumor Cells In this preliminary effort, we use a commercially available and chemically modified tube to selectively capture circulating tumor cells (CTCs) from the blood stream by immobilizing human anti-EpCAM antibodies on the tube's interior surface. We describe the requisite and critical steps required to modify a tube into a cancer cell-capturing device. Using these simple modifications, we were able (...) to capture or entrap about 85% of cancer cells from suspension and 44% of cancer cells from spiked whole blood. We also found that the percentage of cells captured was dependent on the tube's length and also the number of cancer cells present. It is our strong belief that with the utilization of appropriate tube lengths and procedures, we can ensure capture and removal of nearly the entire CTC population in whole blood. Importantly after a patient's entire blood volume has circulated through the tube

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2015 PloS one

49. Risks in Handling of Gastrostomy Tubes

Risks in Handling of Gastrostomy Tubes ¦ Medical Safety Information Pharmaceuticals and Medical Devices Agency http://www.pmda.go.jp/english/service/medical_info.html No. 43 March, 2014 Key points for safe use Risks in Handling of Gastrostomy Tubes (Case) After replacing the gastrostomy tube and infusing nutrients, the patient started complaining about abdominal pain. His face turned pale, and blood pressure decreased. Peritonitis caused by an accidental dislodgement of the gastrostomy tube (...) was suspected. 1 Precautions to prevent gastrostomy tube dislodgement Make sure to confirm proper position of the gastrostomy tubes in the stomach after replacement. Several cases of peritonitis have been reported including fatal cases. The cases were accidentally infused nutrients into the peritoneal cavity due to dislodgment of the gastrostomy tubes. Gastrostomy tube Pallor Facial Respiratory distress Abdominal pain Pyrexia Cold sweat Symptoms of which peritonitis is suspected Enteral nutrition No. 43

2014 Pharmaceuticals and Medical Devices Agency, Japan

50. Management of Blood Borne Viruses within the Haemodialysis Unit

of HCV through internal pathways of modern single-pass dialysis machines has not been demonstrated (KDIGO Hepatitis C Guidelines 3.1). Transmission would require the virion to cross the intact dialyser membrane, migrate from the drain tubing to the fresh dialysate circuit and pass through the dialyzer membrane of a second patient, although the virus cannot cross the intact membrane. Even in the event of a blood leak, transmission would require HCV to reach fresh dialysate used for a subsequent (...) : How frequent is contamination of external pressure monitor filters with blood or saline observed during haemodialysis sessions and what are the factors associated with contamination? Rationale Transducers serve an important role in monitoring the pressures within the arterial and venous circuits. Transducer filter protectors act as a barrier between the blood in the tubing and the internal transducer in the machine. Haemodialysis machines usually have both external (typically supplied

2019 Renal Association

51. Ovarian Epithelial, Fallopian Tube, and Primary Peritoneal Cancer Treatment (PDQ®): Health Professional Version

Ovarian Epithelial, Fallopian Tube, and Primary Peritoneal Cancer Treatment (PDQ®): Health Professional Version Ovarian Epithelial, Fallopian Tube, and Primary Peritoneal Cancer Treatment (PDQ®) - PDQ Cancer Information Summaries - NCBI Bookshelf Warning: The NCBI web site requires JavaScript to function. Search database Search term Search NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health. PDQ Cancer Information Summaries [Internet]. Bethesda (MD (...) ): National Cancer Institute (US); 2002-. PDQ Cancer Information Summaries [Internet]. Bethesda (MD): ; 2002-. Search term Ovarian Epithelial, Fallopian Tube, and Primary Peritoneal Cancer Treatment (PDQ®) Health Professional Version PDQ Adult Treatment Editorial Board . Published online: February 1, 2019. This PDQ cancer information summary for health professionals provides comprehensive, peer-reviewed, evidence-based information about the treatment of ovarian epithelial, fallopian tube, and primary

2018 PDQ - NCI's Comprehensive Cancer Database

52. Ovarian Epithelial, Fallopian Tube, and Primary Peritoneal Cancer Treatment (PDQ®): Patient Version

and ovaries. The doctor or nurse also inserts a lubricated, gloved finger into the rectum to feel for lumps or abnormal areas. Pelvic exam. A doctor or nurse inserts one or two lubricated, gloved fingers of one hand into the vagina and presses on the lower abdomen with the other hand. This is done to feel the size, shape, and position of the uterus and ovaries. The vagina, cervix, fallopian tubes, and rectum are also checked. : A test that measures the level of CA 125 in the blood. CA 125 is a substance (...) Ovarian Epithelial, Fallopian Tube, and Primary Peritoneal Cancer Treatment (PDQ®): Patient Version Ovarian Epithelial, Fallopian Tube, and Primary Peritoneal Cancer Treatment (PDQ®) - PDQ Cancer Information Summaries - NCBI Bookshelf Warning: The NCBI web site requires JavaScript to function. Search database Search term Search NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health. PDQ Cancer Information Summaries [Internet]. Bethesda (MD): National Cancer

2018 PDQ - NCI's Comprehensive Cancer Database

53. Ovarian, Fallopian Tube, and Primary Peritoneal Cancer Prevention (PDQ®): Health Professional Version

Ovarian, Fallopian Tube, and Primary Peritoneal Cancer Prevention (PDQ®): Health Professional Version Ovarian, Fallopian Tube, and Primary Peritoneal Cancer Prevention (PDQ®) - PDQ Cancer Information Summaries - NCBI Bookshelf Warning: The NCBI web site requires JavaScript to function. Search database Search term Search NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health. PDQ Cancer Information Summaries [Internet]. Bethesda (MD): National Cancer (...) Institute (US); 2002-. PDQ Cancer Information Summaries [Internet]. Bethesda (MD): ; 2002-. Search term Ovarian, Fallopian Tube, and Primary Peritoneal Cancer Prevention (PDQ®) Health Professional Version PDQ Screening and Prevention Editorial Board . Published online: March 1, 2019. This PDQ cancer information summary for health professionals provides comprehensive, peer-reviewed, evidence-based information about ovarian, fallopian tube, and primary peritoneal cancer prevention. It is intended

2018 PDQ - NCI's Comprehensive Cancer Database

54. Preanalytical Nonconformity Management Regarding Primary Tube Mixing in Brazil (PubMed)

Preanalytical Nonconformity Management Regarding Primary Tube Mixing in Brazil The multifaceted clinical laboratory process is divided in three essential phases: the preanalytical, analytical and postanalytical phase. Problems emerging from the preanalytical phase are responsible for more than 60% of laboratory errors. This report is aimed at highlighting and discussing nonconformity (e.g., nonstandardized procedures) in primary blood tube mixing immediately after blood collection (...) by venipuncture with evacuated tube systems.From January 2015 to December 2015, fifty different laboratory quality managers from Brazil were contacted to request their internal audit reports on nonconformity regarding primary blood tube mixing immediately after blood collection by venipuncture performed using evacuated tube systems.A minority of internal audits (i.e., 4%) concluded that evacuated blood tubes were not accurately mixed after collection, whereas more than half of them reported that evacuated

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2017 Journal of medical biochemistry

55. Ovarian, Fallopian Tube, and Primary Peritoneal Cancer Screening (PDQ®): Health Professional Version

Ovarian, Fallopian Tube, and Primary Peritoneal Cancer Screening (PDQ®): Health Professional Version Ovarian, Fallopian Tube, and Primary Peritoneal Cancer Screening (PDQ®) - PDQ Cancer Information Summaries - NCBI Bookshelf Warning: The NCBI web site requires JavaScript to function. Search database Search term Search NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health. PDQ Cancer Information Summaries [Internet]. Bethesda (MD): National Cancer Institute (...) (US); 2002-. PDQ Cancer Information Summaries [Internet]. Bethesda (MD): ; 2002-. Search term Ovarian, Fallopian Tube, and Primary Peritoneal Cancer Screening (PDQ®) Health Professional Version PDQ Screening and Prevention Editorial Board . Published online: March 22, 2019. This PDQ cancer information summary for health professionals provides comprehensive, peer-reviewed, evidence-based information about ovarian, fallopian tube, and primary peritoneal cancer screening. It is intended as a resource

2018 PDQ - NCI's Comprehensive Cancer Database

56. Blood Collection Tube

Blood Collection Tube Blood Collection Tube Toggle navigation Brain Head & Neck Chest Endocrine Abdomen Musculoskeletal Skin Infectious Disease Hematology & Oncology Cohorts Diagnostics Emergency Findings Procedures Prevention & Management Pharmacy Resuscitation Trauma Emergency Procedures Ultrasound Cardiovascular Emergencies Lung Emergencies Infectious Disease Pediatrics Neurologic Emergencies Skin Exposure Miscellaneous Abuse Cancer Administration 4 Blood Collection Tube Blood Collection (...) Tube Aka: Blood Collection Tube From Related Chapters II. Preparations: Tubes without Anticoagulant Red Top Tube Red (Barrier Vacuum Tube) III. Preparations: Tubes with Anticoagulant Purple Top Tube (Lavender) Blue Top Tube Green Top Tube Grey Top Tube Yellow Top Tube Navy Blue Top Tube (Royal Blue Top Tube) Images: Related links to external sites (from Bing) These images are a random sampling from a Bing search on the term "Blood Collection Tube." Click on the image (or right click) to open

2015 FP Notebook

57. Peripheral blood gene expression: it all boils down to the RNA collection tubes (PubMed)

Peripheral blood gene expression: it all boils down to the RNA collection tubes Gene expression profiling from peripheral blood is a valuable tool for biomarker discovery in clinical studies. Different whole blood RNA collection and processing methods are highly variable and might confound comparisons of results across studies. The main aim of the study was to compare genome-wide gene expression profiles obtained from the two widely used commercially available whole blood RNA collection systems (...) - PAXgene™ and Tempus™ tubes. Comparisons of present call rates, variances, correlations and influence of globin reduction across the two collection systems was performed using in vivo glucocorticoid stimulation in 24 peripheral blood samples from three individuals.RNA quality, yield and numbers of detected transcripts from the two RNA collection systems was comparable, with no significant differences between the tube types. Globin reduction resulted in a significant increase in present call rates (p

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2012 BMC research notes

58. It’s Time for a Better Blood Collection Tube to Improve the Reliability of Glucose Results (PubMed)

It’s Time for a Better Blood Collection Tube to Improve the Reliability of Glucose Results 23264303 2013 06 06 2018 11 13 1935-5548 36 1 2013 Jan Diabetes care Diabetes Care It's time for a better blood collection tube to improve the reliability of glucose results. e2 10.2337/dc12-1312 Peake Michael J MJ Bruns David E DE Sacks David B DB Horvath Andrea R AR eng Letter United States Diabetes Care 7805975 0149-5992 IY9XDZ35W2 Glucose IM Blood Chemical Analysis instrumentation methods Blood (...) Preservation instrumentation methods Blood Specimen Collection instrumentation methods Glucose metabolism Humans Specimen Handling methods 2012 12 25 6 0 2012 12 25 6 0 2013 6 7 6 0 ppublish 23264303 36/1/e2 10.2337/dc12-1312 PMC3526230 Clin Chim Acta. 1988 Feb 29;172(1):101-8 2966020 Clin Chem. 2008 May;54(5):930-2 18443184 Diabetes Care. 2011 Jun;34(6):e61-99 21617108 Clin Chem. 2009 May;55(5):1019-21 19282354 Clin Chem. 2009 May;55(5):850-2 19282352

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2012 Diabetes Care

59. Blood Collection Tubes

Blood Collection Tubes Blood Collection Tubes Toggle navigation Brain Head & Neck Chest Endocrine Abdomen Musculoskeletal Skin Infectious Disease Hematology & Oncology Cohorts Diagnostics Emergency Findings Procedures Prevention & Management Pharmacy Resuscitation Trauma Emergency Procedures Ultrasound Cardiovascular Emergencies Lung Emergencies Infectious Disease Pediatrics Neurologic Emergencies Skin Exposure Miscellaneous Abuse Cancer Administration 4 '); } document.write(' '); //]]> Blood (...) Collection Tubes Blood Collection Tubes Aka: Blood Collection Tubes From Related Chapters I. Tubes without Anticoagulant Red Top Tube Red (Barrier Vacuum Tube) II. Tubes with Anticoagulant Purple Top Tube (Lavender) Blue Top Tube Green Top Tube Grey Top Tube Yellow Top Tube Navy Blue Top Tube (Royal Blue Top Tube) '); } document.write(' '); //]]> Images: Related links to external sites (from Google) These images are a real-time random sampling from a Google search on the term "Blood Collection Tubes

2015 FP Notebook

60. Blood sample tube transporting system versus point of care technology in an emergency department; effect on time from collection to reporting? A randomised trial. (PubMed)

Blood sample tube transporting system versus point of care technology in an emergency department; effect on time from collection to reporting? A randomised trial. Time is a crucial factor in an emergency department and the effectiveness of diagnosing depends on, among other things, the accessibility of rapid reported laboratory test results; i.e.: a short turnaround time (TAT). Former studies have shown a reduced time to action when point of care technologies (POCT) are used in emergency (...) departments. This study assesses the hypothesis, that using Point of Care Technology in analysing blood samples versus tube transporting blood samples for laboratory analyses results in shorter time from the blood sample is collected to the result is reported in an emergency department.The study was designed as a randomised controlled trial with consecutive allocation into two groups and rated 1:1. Blood samples were collected on all included patients and then randomised into either POCT analyses or tube

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2012 Scandinavian journal of trauma, resuscitation and emergency medicine Controlled trial quality: uncertain

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