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Blood Collection Tube

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181. Improving the timeframe between blood collection and interferon gamma release assay using T-Cell Xtend(®). (PubMed)

Improving the timeframe between blood collection and interferon gamma release assay using T-Cell Xtend(®). Vacutainer CPT tubes require blood samples for TSPOT.TB to be processed within 8 h. In this study we evaluated the ability of T-Cell Xtend to maintain the number and function of lymphocytes after 24 and 48 h of blood storage, giving similar test results as in freshly isolated specimens.Whole blood specimens from 59 individuals were collected in Vacutainer CPT tubes (CPT) and lithium (...) heparin (LH) tubes. CPT tubes were processed within 8 h. T-Cell Xtend was added to LH tubes after 24 or 48 h. We also left LH tubes untreated for 48 h. Total number of white blood cells (WBC) and proportions of lymphocytes and granulocytes were determined in the isolated Peripheral Blood Mononuclear Cells (PBMC). We also evaluated the performance of T-Cell Xtend in the TSPOT.TB assay.PBMC yields from T-Cell Xtend treated LH samples did not differ from PBMC yields from CPT tubes, but T-Cell Xtend had

2011 Journal of Infection

182. Observations on the collection and handling of blood samples for N.E.F.A. estimation (PubMed)

Observations on the collection and handling of blood samples for N.E.F.A. estimation The effect upon the results of estimating plasma or serum non-esterified fatty acids (N.E.F.A.) assay by different methods of collecting blood samples and subsequent differences in sample storage and time of assay have been studied. Standardization of technique is advised and the use of chilled heparinized tubes, with separation of the sample within one hour of collection and assay on the day of collection

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1967 Journal of Clinical Pathology

183. Flow Cytometric Analysis of Peripheral Blood Neutrophil Myeloperoxidase Expression and Myelodysplastic Syndromes

in peripheral blood samples will be performed within 24 h of MDS diagnostic evaluation and blinded to the reference standard. Peripheral blood samples will be collected in 5 ml (EDTA) anticoagulant plastic tubes and processed within 24 h maximum of collection. The samples will be stored at 4°C overnight. Blood samples containing at least 105 neutrophils will be incubated with a panel of antibodies conjugated to fluorochromes, according to the manufacturers' recommendations. At least 10,000 cell events (...) Flow Cytometric Analysis of Peripheral Blood Neutrophil Myeloperoxidase Expression and Myelodysplastic Syndromes Flow Cytometric Analysis of Peripheral Blood Neutrophil Myeloperoxidase Expression and Myelodysplastic Syndromes - Full Text View - ClinicalTrials.gov Hide glossary Glossary Study record managers: refer to the if submitting registration or results information. Search for terms x × Study Record Detail Saved Studies Save this study Warning You have reached the maximum number of saved

2017 Clinical Trials

184. T790M Mutation Testing in Blood by Different Methodologies

blood samples per patient will be collected once at the time of progression, assessed by CT Scans according to RECIST criteria v.1.1 and before the patients start a new treatment The blood samples (5-10 mL each) will be collected in one Cell-Free DNA BCT Streck® and 2 PTT EDTA K2 (BECTON DICKINSON) collection tubes. All samples will be labeled properly with the patient identification number and date of extraction. These samples will be stored and distributed through the 3 participating laboratories (...) in Spain for T790M identification in circulating-free DNA isolated from blood collected at the time of progression on a first or second generation TKI Secondary Outcome Measures : Cost of the different methodologies [ Time Frame: At 12 months from the first inclusion ] To compare the cost of the different methodologies Specificity and sensitivity of each cfDNA method [ Time Frame: At 12 months from the first inclusion ] To estimate the specificity and sensitivity of each cfDNA method. Other Outcome

2017 Clinical Trials

185. Mathematical Arterialization of Venous Blood Gas

Reliable Compared to Arterial Blood Gas? - a Clinical Validation Study Actual Study Start Date : September 1, 2015 Actual Primary Completion Date : January 30, 2016 Actual Study Completion Date : October 1, 2017 Groups and Cohorts Go to Group/Cohort Intervention/treatment Pre-study In the pre-study, venous samples were collected in paired 2 mL ABG syringes and 4.5 mL tubes from each of the 10 patients, to determine which blood collection method was preferred. VBG samples were collected via a butterfly (...) needle with a three-way stopcock in conjunction with routine venous blood sampling upon admission. VBG samples were collected by the biomedical laboratory technician in the same manner as PVB samples in the normal clinical setting. Results from the pre-study were used to determine the preferred blood collection method in the main study. In this study, paired ABG and VBG samples were collected simultaneously from each of the 20 patients. The ABG samples were collected by the responsible physician

2017 Clinical Trials

186. Role of Glycaemic Index and High Protein Meal in Response of Blood Biomarkers for Pre-diabetes

will arrived between 8.00am and 8.30am the following morning. Baseline blood samples will be collected. A venous cannula will be inserted and a fasting blood sample collected. Baseline basal metabolic rate will be measured using hood-ventilated indirect calorimetry for 30mins during fasting. Subjects will then consume the test meal. Further venous blood samples will be collected at regular intervals for up to 4hours postprandial. After meal, subjects will enter the hood-ventilated indirect calorimetry (...) Role of Glycaemic Index and High Protein Meal in Response of Blood Biomarkers for Pre-diabetes Role of Glycaemic Index and High Protein Meal in Response of Blood Biomarkers for Pre-diabetes - Full Text View - ClinicalTrials.gov Hide glossary Glossary Study record managers: refer to the if submitting registration or results information. Search for terms x × Study Record Detail Saved Studies Save this study Warning You have reached the maximum number of saved studies (100). Please remove one

2017 Clinical Trials

187. Decreasing Postoperative Blood Loss by Topical vs. Intravenous Tranexamic Acid in Open Cardiac Surgery

excessive blood loss from major trauma, post partum, surgery, tooth removal, nose bleeds, and heavy menstruation. Other Name: Cyklokapron Outcome Measures Go to Primary Outcome Measures : Postoperative chest tube production 24 hours after surgical procedure [ Time Frame: 24 hours after surgical procedure ] Will be analyzed with a t-test as it is a continuous variable Secondary Outcome Measures : Postoperative seizure development [ Time Frame: Seizures will be documented from date of surgery to hospital (...) Decreasing Postoperative Blood Loss by Topical vs. Intravenous Tranexamic Acid in Open Cardiac Surgery Decreasing Postoperative Blood Loss by Topical vs. Intravenous Tranexamic Acid in Open Cardiac Surgery - Full Text View - ClinicalTrials.gov Hide glossary Glossary Study record managers: refer to the if submitting registration or results information. Search for terms x × Study Record Detail Saved Studies Save this study Warning You have reached the maximum number of saved studies (100). Please

2017 Clinical Trials

188. Seroepidemiology of Human Brucellosis Among Blood Donors in Southern Ethiopia: Calling Attention to a Neglected Zoonotic Disease. (PubMed)

samples were collected to screen for serum agglutinins reactive to stained antigen of Brucella abortus Standard tube titration was performed for reactive serum to determine the titer of the agglutinin. A structured questionnaire was used to collect data on possible risk factors for brucellosis. The seroprevalence of human brucellosis in this study was found to be 10.6% (95% confidence interval = 7.0, 14.0). Possession of domestic ruminant animals, contact with ruminant animals, and husbandry practices (...) Seroepidemiology of Human Brucellosis Among Blood Donors in Southern Ethiopia: Calling Attention to a Neglected Zoonotic Disease. Human brucellosis is neglected in southern Ethiopia. Although traditional food processing practices and animal husbandry which increase the risk of brucellosis are common, it has not been properly studied yet. This study was conducted to determine the seroepidemiology of brucellosis among apparently healthy individuals in southern Ethiopia. In the study, blood

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2017 American Journal of Tropical Medicine & Hygiene

189. 'Future-proofing' blood processing for measurement of circulating microRNAs in samples from biobanks and prospective clinical trials. (PubMed)

blood-derived specimens, plasma samples in EDTA, Streck-DNA, and Streck-RNA tubes showed significantly higher Ct values for multiple housekeeping miRNAs, compared with serum samples. For the EDTA-plasma tubes, this difference was only seen when including the high-speed centrifugation protocol used to optimize ctDNA extraction. In plasma samples derived from blood stored at room temperature for up to 14 days (conditions that typically apply to samples processed for biobanking), levels of endogenous (...) 'Future-proofing' blood processing for measurement of circulating microRNAs in samples from biobanks and prospective clinical trials. Background: Quantifying circulating nucleic acids is an important new approach to cancer diagnosis/monitoring.Methods: We compared the suitability of serum versus plasma for measuring miRNAs using qRT-PCR and assessed how preanalytic variables that can affect circulating tumor DNA (ctDNA) quantification in plasma also influence miRNA levels.Results: Across 62

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2017 Cancer Epidemiology & Biomarkers and Prevention

190. Evaluation of Existing Methods for Human Blood mRNA Isolation and Analysis for Large Studies. (PubMed)

and extraction kits caused strong variations in gene expression. The use of PAXgeneTM and TempusTM collection systems resulted in RNA of good quality and quantity for the respective RNA isolation system. No large inter-donor variations could be detected for both systems. However, it was not possible to extract sufficient RNA of good quality with the PAXgeneTM RNA extraction system from samples collected by TempusTM collection tubes. Comparing only the Norgen RNA extraction methods, RNA from blood collected (...) Evaluation of Existing Methods for Human Blood mRNA Isolation and Analysis for Large Studies. Prior to implementing gene expression analyses from blood to a larger cohort study, an evaluation to set up a reliable and reproducible method is mandatory but challenging due to the specific characteristics of the samples as well as their collection methods. In this pilot study we optimized a combination of blood sampling and RNA isolation methods and present reproducible gene expression results from

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2016 PLoS ONE

191. Feasibility and Safety of Umbilical Cord Blood Transfusion in the Treatment of Neonatal Cerebral Ischemia and Anemia

infants with presence of clinical indications of neonatal hypoxic-ischemia encephalopathy (HIE) and anemia. Umbilical cord blood (UCB) is collected following labor and is transfused intravenously within 48 hours after the birth. Newborn infant without UCB available recieves the standard care will be enrolled as control group. Following the autologous UCB transfusion in the study group or standard care in the control group, HIE subjects will be followed for 2 years for survival and neurodevelopmental (...) Feasibility and Safety of Umbilical Cord Blood Transfusion in the Treatment of Neonatal Cerebral Ischemia and Anemia Feasibility and Safety of Umbilical Cord Blood Transfusion in the Treatment of Neonatal Cerebral Ischemia and Anemia - Full Text View - ClinicalTrials.gov Hide glossary Glossary Study record managers: refer to the if submitting registration or results information. Search for terms x × Study Record Detail Saved Studies Save this study Warning You have reached the maximum number

2017 Clinical Trials

192. Blood Test for Early Detection of Breast Cancer Using Todos Medical -Breast 1(TM-B1) Assay

), evaluate the performance, in terms of sensitivity and specificity, of the TM-B1 test and XRM together as a breast cancer screening procedure compared to the XRM alone [ Time Frame: Timepoint one, day 1 ] The subject's blood (Groups 1 and 2) will be tested for the presence of breast cancer Biospecimen Retention: Samples Without DNA Collect blood from the subject in two 6 ml K2 EDTA blood collection tube (total 12 ml blood) according to standardized phlebotomy procedures. Eligibility Criteria Go (...) Posted : November 17, 2017 Last Update Posted : August 22, 2018 See Sponsor: Todos Medical, Ltd. Information provided by (Responsible Party): Todos Medical, Ltd. Study Details Study Description Go to Brief Summary: Blood samples from a total of 200 women in two cohorts will be collected and analyzed by TM-B1 assay, which is based on TBIA (Total Biochemical Infrared Analysis) to confirm the presence of cancer. These two cohorts will yield 75 healthy women with no pathological findings, 75 women

2017 Clinical Trials

193. Blood-Brain Barrier Disruption in People With White Matter Hyperintensities Who Have Had a Stroke

Blood-Brain Barrier Disruption in People With White Matter Hyperintensities Who Have Had a Stroke Blood-Brain Barrier Disruption in People With White Matter Hyperintensities Who Have Had a Stroke - Full Text View - ClinicalTrials.gov Hide glossary Glossary Study record managers: refer to the if submitting registration or results information. Search for terms x × Study Record Detail Saved Studies Save this study Warning You have reached the maximum number of saved studies (100). Please remove (...) one or more studies before adding more. Blood-Brain Barrier Disruption in People With White Matter Hyperintensities Who Have Had a Stroke The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. of clinical studies and talk to your health care provider before participating. Read our for details. ClinicalTrials.gov Identifier: NCT03366129 Recruitment Status

2017 Clinical Trials

194. A methodological consideration for blood lead concentrations obtained from the earlobe in Japanese adults occupationally unexposed to lead (PubMed)

vein.Paired blood samples were collected from the earlobe and cubital vein of 112 Japanese participants occupationally unexposed to lead, and the BPb levels were determined using ICP-MS.The limit of detection of BPb for the ICP-MS method was 0.015 μg/dL, and there was no participant with a BPb level below this limit. The median values of BPb concentrations were 0.91 (range, 0.41-2.48) μg/dL for earlobe blood using a 175-μL capillary tube and 0.85 (0.35-2.39) μg/dL for venous blood using a 5-mL vacuum tube (...) . There was a significant correlation between the earlobe BPb levels and cubital vein BPb levels (Spearman rank correlation r S = 0.941), though the earlobe BPb levels were significantly higher than the cubital vein BPb levels. Most of the participants regarded earlobe puncture as a painless method.These data suggest that earlobe BPb levels can be used to assess lead exposure in children. Blood collection using a capillary tube should be done carefully and promptly because slow withdrawal may lead to measurement bias.

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2017 Environmental health and preventive medicine

195. Fresh Autologous Whole Blood Transfusion After Cardiopulmonary Bypass

may be life-saving, significant risks of complications such as lung injury or even an increase in mortality are associated with transfusion. Decreasing transfusion requirements during cardiac surgery has the potential to reduce the rate of complications, improve patient outcomes, and reduce cost resulting in increased value for both the patient and the health system as a whole. Collection of autologous blood before cardiopulmonary bypass (CPB) for transfusion after CPB has been shown to be both (...) blood collection prior to CPB for transfusion post-operatively has been shown to improve coagulation and decrease clot lysis but is not routinely performed because 90% to 95% of patients do not have extensive blood loss and subsequent coagulopathy. Coupling accurate pre-operative bleeding risk prediction with autologous fresh whole blood collection for transfusion after CPB would target an established, low cost, low risk intervention to an at risk patient population who may experience significant

2017 Clinical Trials

196. Tea Extract, Blood Lipid and Dietary Fat

one hour before testing. Upon arrival at the laboratory a person trained and experienced in sampling blood will insert a cannula (a small plastic tube) into a vein on the participants arm. This is to allow for repeated blood sampling every 30-60 minutes during their visit. Participants will then be provided with one of the four supplements under investigation (i.e. either a placebo or one of the preparations derived from tea) to ingest as fluid along with a standard milkshake that provides (...) : Placebo Participants will consume 567 ml of plain water at least 1 hour before testing the trial. Information on dietary compliance will be collected. Participants will then be cannulated in an arm vein and a baseline fasting blood sample will be taken. Participants will be asked to consume a standard study meal challenge (providing 40 g dietary fat and less than 2 g carbohydrate or 1.2 g protein) and placebo drink within 15 min. Serial blood samples will be collected at baseline, 30, 60, 90, 120

2017 Clinical Trials

197. Blood microRNA Depression Change After DHCA

Description Go to Brief Summary: Collect the blood sample per-operation experience deep hypothermia cardiac arrest for aorta artery replacement surgery Collect the blood from the CPB machine and from intravenous injection tube which already set at different time point. Condition or disease Intervention/treatment Aortic Aneurysm Other: collect blood Detailed Description: Collect the blood sample per-operation experience deep hypothermia cardiac arrest for aorta artery replacement surgery Collect the blood (...) from the CPB machine and from intravenous injection tube which already set at different time point Test the blood sample of DHCA, analysis the correlation between miR-194 and temperature. Study Design Go to Layout table for study information Study Type : Observational Actual Enrollment : 21 participants Observational Model: Cohort Time Perspective: Prospective Official Title: Blood microRNA Depression Change After DHCA Actual Study Start Date : December 1, 2016 Actual Primary Completion Date

2017 Clinical Trials

198. TIVA and VIMA on Blood Glucose and Insulin Levels in Lung Lobectomy Patients

intramuscularly. Immediately after arrival in the operating room, blood pressure, electrocardiogram and oxygen saturation are measured and monitored, and a bispectral index (BIS) is attached to the patient's forehead to determine the appropriate anesthetic depth during surgery. A 22G angiocatheter is inserted into the radial artery after local anesthesia with lidocaine to monitor the blood pressure in real time and to collect blood for arterial blood gas analysis during one side of pulmonary ventilation (...) TIVA and VIMA on Blood Glucose and Insulin Levels in Lung Lobectomy Patients TIVA and VIMA on Blood Glucose and Insulin Levels in Lung Lobectomy Patients - Full Text View - ClinicalTrials.gov Hide glossary Glossary Study record managers: refer to the if submitting registration or results information. Search for terms x × Study Record Detail Saved Studies Save this study Warning You have reached the maximum number of saved studies (100). Please remove one or more studies before adding more. TIVA

2017 Clinical Trials

199. Drinking Water Salinity and Raised Blood Pressure: Evidence from a Cohort Study in Coastal Bangladesh (PubMed)

the results of a cohort study in which we assessed the effects of drinking-water sodium (DWS) on blood pressure (BP) in coastal populations in Bangladesh.DWS, BP, and information on personal, lifestyle, and environmental factors were collected from 581 participants. We used generalized linear latent and mixed methods to model the effects of DWS on BP and assessed the associations between changes in DWS and BP when participants experienced changing sodium levels in water, switched from "conventional" ponds (...) Drinking Water Salinity and Raised Blood Pressure: Evidence from a Cohort Study in Coastal Bangladesh Millions of coastal inhabitants in Southeast Asia have been experiencing increasing sodium concentrations in their drinking-water sources, likely partially due to climate change. High (dietary) sodium intake has convincingly been proven to increase risk of hypertension; it remains unknown, however, whether consumption of sodium in drinking water could have similar effects on health.We present

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2017 Environmental health perspectives

200. Noncontact 3-dimensional Speckle Contrast Diffuse Correlation Tomography of Tissue Blood Flow Distribution (PubMed)

using tissue phantoms with a pump-connected tube (high) and phantom spheres (low). Furthermore, in vivo validation of extracting spatiotemporal 3-D blood flow distributions and hyperemic response during forearm cuff occlusion is demonstrated. Finally, the success of instrument feasibility in clinical use is examined through the intraoperative imaging of mastectomy skin flap. (...) Noncontact 3-dimensional Speckle Contrast Diffuse Correlation Tomography of Tissue Blood Flow Distribution Recent advancements in near-infrared diffuse correlation techniques and instrumentation have opened the path for versatile deep tissue microvasculature blood flow imaging systems. Despite this progress there remains a need for a completely noncontact, noninvasive device with high translatability from small/testing (animal) to large/target (human) subjects with trivial application on both

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2017 IEEE transactions on medical imaging

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