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Blood Collection Tube

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181. Maternal Serum BLyS Levels Throughout Pregnancy

as 28 0/7 gestational weeks to delivery. Postpartum samples were collected before hospital discharge after delivery. Blood samples were collected in sterile tubes without additives and refrigerated until processing. Sera were routinely isolated from the clotted blood samples within 12 to 24 hours and were aliquotted and stored at −80°C. Serum BAFF levels were measured in a blinded fashion using a sandwich ELISA. Chi-squared or Fisher exact testing was used for categorical variables, and Kruskal (...) hypertension that was accompanied by proteinuria of at least 300 mg in a 24-hour urine collection or a protein/creatinine ratio ≥0.3. HELLP syndrome was diagnosed when a woman had laboratory evidence of hemolysis, a platelet count <100,000/μL, and serum levels of aspartate aminotransferase and alanine aminotransferase that were greater than or equal to twice normal values. Superimposed preeclampsia was diagnosed in a participant with CHTN when the participant experienced an acute exacerbation

2017 Clinical Trials

182. Multiple soluble TGF-β receptors in addition to soluble endoglin are elevated in preeclamptic serum and they synergistically inhibit TGF-β signalling. Full Text available with Trip Pro

, with a low affinity to TGF-β ligands, has been demonstrated to have little effect by itself on TGF-β action.We examined whether multiple soluble TGF-β receptors are elevated in PE circulation and whether they synergistically block TGF-β signaling.TGF-β receptors were measured using enzyme-linked immunosorbent assay in sera collected from preeclamptic pregnancies and gestation-age-matched controls. TGF-β signaling was assessed using an in vitro bioassay and a tube formation assay.TGF-β type I, II, and III (...) Multiple soluble TGF-β receptors in addition to soluble endoglin are elevated in preeclamptic serum and they synergistically inhibit TGF-β signalling. Preeclampsia (PE) can be classified into early-onset (<34 weeks of gestation) and late-onset (>34 weeks of gestation) subtypes. Soluble endoglin, an auxiliary receptor for transforming growth factor (TGF)-β ligands, is increased in PE circulation and believed to inhibit TGF-β action by sequestering the ligands. However, soluble endoglin

2017 Journal of Clinical Endocrinology and Metabolism

183. Observations on the collection and handling of blood samples for N.E.F.A. estimation Full Text available with Trip Pro

Observations on the collection and handling of blood samples for N.E.F.A. estimation The effect upon the results of estimating plasma or serum non-esterified fatty acids (N.E.F.A.) assay by different methods of collecting blood samples and subsequent differences in sample storage and time of assay have been studied. Standardization of technique is advised and the use of chilled heparinized tubes, with separation of the sample within one hour of collection and assay on the day of collection

1967 Journal of Clinical Pathology

184. Stability of Circulating Blood-Based MicroRNAs - Pre-Analytic Methodological Considerations. Full Text available with Trip Pro

. The aim of this study was to investigate the stability of miRNAs in blood under various relevant clinical and research conditions: different collection tubes, storage at different temperatures, physical disturbance, as well as serial freeze-thaw cycles.Blood samples were collected from 12 healthy donors into different collection tubes containing anticoagulants, including EDTA, citrate and lithium-heparin, as well as into serum collection tubes. MiRNA stability was evaluated by measuring expression (...) months) whole blood samples were significantly changed, which is in contrast to the plasma samples, where miR-21 or miR-29b levels were found to be stable. Repetitive (n = 4) freeze-thaw cycles resulted in a significant reduction of miRNA concentration both in plasma and serum samples.This study highlights the importance of proper and systematic sample collection and preparation when measuring circulating miRNAs, e.g., in context of clinical trials. We demonstrated that the type of collection tubes

2017 PLoS ONE

185. Comparison of heel stick sample with placental blood sample for pretransfusion testing. (Abstract)

testing potentially reduces the amount of blood collected from newborns for initial laboratory tests.Placental samples were collected for pretransfusion tests at birth from 32 newborns with less than 2000 g birthweight and less than 35 weeks to compare the results with the heel stick samples from the same newborns. ABO and D typing, direct antiglobulin test (DAT) with IgG, and antibody screen tests were performed on these sample pairs. For ABO and D typing both tube and gel methods were used (...) Comparison of heel stick sample with placental blood sample for pretransfusion testing. Published studies demonstrate that placental blood samples provide acceptable results for various laboratory tests, but these studies did not include pretransfusion testing. The aim of this study was to show concordance between heel stick and placental blood sample pairs for pretransfusion testing and to validate tube and gel method for placental samples. Using placental blood samples for pretransfusion

2018 Transfusion

186. Haplo-Identical Transplantation for Severe Aplastic Anemia and Hypo-Plastic MDS Using Peripheral Blood Stem Cells and Post-Transplant Cyclophosphamide for GVHD Prophylaxis

blood hematopoietic stem cells from a family member plus chemotherapy. Eligibility: Recipients ages 4 55 with SAA or MD and their relative donors ages 4 75 Design: Recipients will have: Blood, urine, heart, and lung tests Scans Bone marrow sample Recipients will need a caregiver for several months. They may make fertility plans and a power of attorney. Donors will have blood and tissue tests, then injections to boost stem cells for 5 7 days. Donors will have blood collected from a tube in an arm (...) ) ) Study Details Study Description Go to Brief Summary: Background: Severe aplastic anemia (SAA) and myelodysplastic syndrome (MD) cause serious blood problems. Stem cell transplants using bone marrow or blood plus chemotherapy can help. Researchers want to see if using peripheral blood stem cells (PBSCs) rather than bone marrow cells works too. PBSCs are easier to collect and have more cells that help transplants. Objectives: To see how safely and effectively SAA and MD are treated using peripheral

2018 Clinical Trials

187. Addition of microRNA Blood Test to Lung Cancer Screening Low Dose CT

available diagnostic information (LDCT, subsequent diagnostic imaging studies, biopsy results and surgical pathology results) obtained during at least 12 months of follow-up. Biospecimen Retention: Samples With DNA Blood will be drawn from all patients at the time of study enrollment prior to LDCT scanning (10cc blood divided into 2 PAXgene tubes) Eligibility Criteria Go to Information from the National Library of Medicine Choosing to participate in a study is an important personal decision. Talk (...) Addition of microRNA Blood Test to Lung Cancer Screening Low Dose CT Addition of microRNA Blood Test to Lung Cancer Screening Low Dose CT - Full Text View - ClinicalTrials.gov Hide glossary Glossary Study record managers: refer to the if submitting registration or results information. Search for terms x × Study Record Detail Saved Studies Save this study Warning You have reached the maximum number of saved studies (100). Please remove one or more studies before adding more. Addition of microRNA

2018 Clinical Trials

188. Magnetic Resonance (MR) Imaging & Blood Biomarkers for Head and Neck Cancer

-guided radiation therapy (GRT). Participant will complete the quality-of-life questionnaire. Wherever possible, blood samples will be collected at the same time as routine blood draws and/or when a catheter (needle and tube) is placed. However, if a blood draw is not planned, one will be scheduled for this study. Length of Study: Participation on the study will be over after the follow-up visit. Participant will be taken off study early if radiation treatment is stopped early. Follow-up: One (1) time (...) Years and older (Adult, Older Adult) Sexes Eligible for Study: All Accepts Healthy Volunteers: No Criteria Inclusion Criteria: Biopsy proven diagnosis of squamous cell carcinoma (SCC) of head and neck mucosa. Clinical evidence should be documented, and may consist of imaging, endoscopic evaluation, palpation, and should be sufficient to estimate the size of the primary for purposes of T staging. Age ≥18 years No distant metastases, based on routine staging workup. Consent for blood collection

2018 Clinical Trials

189. Comparison Between K3EDTA and Lithium Heparin as Anticoagulant to Isolate Bovine Granulocytes From Blood Full Text available with Trip Pro

of the anticoagulants K3EDTA or lithium heparin and the effect of different RBCs lysis methods on bovine granulocyte population from fresh blood of healthy cows after density gradient centrifugation were investigated. Venous blood from healthy cows was collected in K3EDTA and lithium heparin tubes. Density gradient centrifugation to separate granulocytes from other cells was conducted using Biocoll. Then, RBCs were lysed with hypotonic water or 0.2% sodium chloride (NaCl). Immediately after isolation, harvest (...) Comparison Between K3EDTA and Lithium Heparin as Anticoagulant to Isolate Bovine Granulocytes From Blood Granulocytes play a key role in the defense against invading pathogens. To study granulocyte functions, the isolation of a pure and active cell population from fresh blood is required. Anticoagulants and red blood cells (RBCs) lysis used in the isolation procedure may influence cell harvest, cell marker expression, and pre-activation of cells. In this study, the influence

2018 Frontiers in immunology

190. Reference intervals for a complete blood count on an automated haematology analyser Sysmex XN in healthy adults from the southern metropolitan area of Barcelona Full Text available with Trip Pro

blood count (CBC) on a Sysmex XN haematology analyser in healthy adults from the southern metropolitan area of Barcelona (Spain).A total of 213 apparently healthy adults who received a general health examination at Hospital Universitari de Bellvitge were recruited to this study. Blood samples collected in K3EDTA tubes were analysed on a Sysmex XN. Statistically relevant gender based partition was assessed, outliers removed, and the reference intervals calculated in concordance with Clinical (...) Reference intervals for a complete blood count on an automated haematology analyser Sysmex XN in healthy adults from the southern metropolitan area of Barcelona The examination of peripheral blood is routinely used as a basic test in daily medical practice. Reliable reference intervals are necessary to avoid misdiagnoses, and the establishment of those intervals is an important task for clinical laboratories.The aim of the present study was to establish the reference intervals for complete

2018 EJIFCC

191. Delayed double reading of whole blood clotting test (WBCT) results at 20 and 30 minutes enhances diagnosis and treatment of viper envenomation Full Text available with Trip Pro

Delayed double reading of whole blood clotting test (WBCT) results at 20 and 30 minutes enhances diagnosis and treatment of viper envenomation The whole blood clotting test (WBCT) is a simple test of coagulation that is often used in the assessment, diagnosis, and therapeutic monitoring of snakebite patients in sub-Saharan Africa. WBCT requires only a clean glass tube and several milliliters of venous blood and is ideal for use in poorly equipped health centers throughout the rural areas where (...) collection in 23 patients treated for snake envenomation in Bembèrèkè, northern Benin.WBCT results were identical at both reading times in patients without coagulopathy or when coagulation was restored permanently following a single dose of antivenom. Out of 17 patients with coagulopathy, 14 showed discrepancies between WBCT20 and WBCT30 results in at least one pair of serial evaluations. These could be completely contradictory results (e.g. normal clot at WBCT20 and no clot at WBCT30) or a marked

2018 The journal of venomous animals and toxins including tropical diseases

192. Optimizing recovery of frozen human peripheral blood mononuclear cells for flow cytometry. Full Text available with Trip Pro

Optimizing recovery of frozen human peripheral blood mononuclear cells for flow cytometry. Live peripheral blood mononuclear cells (PBMCs) can be frozen and thawed for later analyses by adding and removing a cryoprotectant, such as dimethyl sulfoxide (DMSO). Laboratories across the world use various procedures, but published evidence of optimal thawing procedures is scarce.PBMCs were separated from blood collected from healthy Danish blood donors, and stored at -80°C after adding of DMSO (...) . The essential steps in the thawing procedure were modified and performance was evaluated by flow cytometry with respect to the percentage and total yield of viable PMBCs.The best-performing washing medium was Roswell Park Memorial Institute (RPMI) 1640 at 37°C with 20% fetal bovine serum. When using 10 mL washing medium in a 15-mL Falcon tube, samples should be centrifuged for at least 10 minutes at 500 g. We failed to detect any differences between the tested methods of mixing PBMCs with washing medium

2017 PLoS ONE

193. Valid Biomarkers in Blood to Predict the Response to Therapy in Prostate Cancer Patients

in EDTA tube for analysis of biological biomarkers in different time points. Condition or disease Intervention/treatment Prostate Cancer Other: biological samples collection and analysis Detailed Description: Participants to the study will be assigned to 5 cohorts according to the stage of disease and planned antitumor treatment at time of study entry. Group 0 (set-up): blood sampling and rectal swab will be collected at a single time-point during the PC treatment. No follow-up is required. Group 1a (...) 24, 2018 Last Update Posted : January 4, 2019 See Sponsor: Andrea Alimonti Collaborator: Clinical Trial Unit Ente Ospedaliero Cantonale Information provided by (Responsible Party): Andrea Alimonti, Oncology Institute of Southern Switzerland Study Details Study Description Go to Brief Summary: Participants to the study will be assigned to 5 cohorts according to the stage of disease and planned antitumor treatment at time of study entry. In all patients, 12 ml of blood sample will be collected

2018 Clinical Trials

194. A Pilot Study- Monitoring Cerebral Blood Flow in Neonates With Congenital Heart Defects

at the same day and one and two days after, for 3 hours per day. Additional standard of care parameters will be collected :Heart rate, Mean Arterial Blood Pressure, Saturation, Ventilator rate, Medications, Blood gases ( blood tests), Echocardiogram, pre-surgery Head US,. additional examination will be performed intended for the study: two post surgery Head US, S100B biomarker for brain injury and Neurological examinations prior and 7 days post- surgery( By standardized questionnaires). Sample size: 55 (...) used to measure relative changes in blood flow. The device monitors regional micro circulatory blood flow in tissues, by using sensors placed near the area of interest. Outcome Measures Go to Primary Outcome Measures : Change in c-Flow3310-P following routine maneuvers [ Time Frame: 3 hours monitoring per day, for 4 days ] Changes will be measured following routine maneuvers such as suction, leg lifting, Blood sample, changing position, feeding, presence of feeding tube etc. Change= (CFI

2018 Clinical Trials

195. Effect of Steroid Administration on Maternal Blood Levels of hLPCAT1 mRNA

with all other relevant clinical labs (in PAX gene tubes) on admission, 24hrs and 48hrs after the course of steroids is initiated. Samples will also be collected 1 and 2 weeks later to see if steroid effect degrades over time. This would have immense clinical implication, especially when it comes to rescue doses. Investigators will also correlate measures of neonatal outcomes (such as apgar scores, blood gases, NICU admission, need to go on respiratory/ventilator support) to the most recent maternal (...) with lamellar body count, a well established clinical marker of fetal lung maturity. Condition or disease Intervention/treatment Fetal Lung Maturity Respiratory Distress Syndrome in Premature Infant Other: blood draw Detailed Description: OBJECTIVES/AIMS Group 1: Determine the serum levels of hLPCAT1 mRNA in pregnant women from 32- 36+6/7 weeks gestation. More specifically, to determine at what moment in gestation, expression spontaneously begins. Group 2: Determine how (and if) serum hLPCAT1 mRNA changes

2018 Clinical Trials

196. HEARTBiT: Multi-Marker Blood Test for Acute Cardiac Transplant Rejection

and NR as ordered variables. Biospecimen Retention: Samples With DNA Whole blood samples to be collected in four (4) tubes (approx. 15mL or 3 teaspoons) consisting of: 1 - 6 mL lavender top (EDTA) tube (plasma) 1 - 4 mL red top tube (serum) 2 - 2.5 mL PAXgene tubes (RNA) Blood processing (fractionation) of plasma and serum tubes following SOP guidelines will result in collection of: 8 - 0.5mL plasma aliquots 1 - 0.5mL buffy coat DNA sample 6 - 0.5mL serum aliquots Eligibility Criteria Go (...) HEARTBiT: Multi-Marker Blood Test for Acute Cardiac Transplant Rejection HEARTBiT: Multi-Marker Blood Test for Acute Cardiac Transplant Rejection - Full Text View - ClinicalTrials.gov Hide glossary Glossary Study record managers: refer to the if submitting registration or results information. Search for terms x × Study Record Detail Saved Studies Save this study Warning You have reached the maximum number of saved studies (100). Please remove one or more studies before adding more. HEARTBiT

2018 Clinical Trials

197. Changes in cytokine responses to TB antigens ESAT-6, CFP-10 and TB 7.7 and inflammatory markers in peripheral blood during therapy Full Text available with Trip Pro

), and at the end of anti-TB treatment (Tend). We measured the levels of Interferon (IFN)-γ, interleukin (IL)-2, IL-12, IL-10, IL-13 and tumor necrosis factor (TNF)-α in supernatants collected from the QuantiFERON-TB Gold In-Tube assay (QFT-GIT), as well as the WBC, neutrophil, platelet count and neutrophil to lymphocyte ratio (NLR) in whole blood. Compared with baseline levels, WBC, neutrophil, and platelet counts were significantly lower following treatment. In addition, the NLR after treatment significantly (...) Changes in cytokine responses to TB antigens ESAT-6, CFP-10 and TB 7.7 and inflammatory markers in peripheral blood during therapy Multiple cytokines and inflammatory markers control TB infection. We aimed to investigate the changes in multiple cytokines and inflammatory markers in active TB patients following anti-TB drug therapy. Twenty-nine patients with active TB were recruited prospectively between December 2010 and July 2017. Blood samples were collected before (T0), after 2 months (T2

2018 Scientific reports

198. Whole blood FPR1 mRNA expression predicts both non‐small cell and small cell lung cancer Full Text available with Trip Pro

Whole blood FPR1 mRNA expression predicts both non‐small cell and small cell lung cancer While long-term survival rates for early-stage lung cancer are high, most cases are diagnosed in later stages that can negatively impact survival rates. We aim to design a simple, single biomarker blood test for early-stage lung cancer that is robust to preclinical variables and can be readily implemented in the clinic. Whole blood was collected in PAXgene tubes from a training set of 29 patients (...) , and a validation set of 260 patients, of which samples from 58 patients were prospectively collected in a clinical trial specifically for our study. After RNA was extracted, the expressions of FPR1 and a reference gene were quantified by an automated one-step Taqman RT-PCR assay. Elevated levels of FPR1 mRNA in whole blood predicted lung cancer status with a sensitivity of 55% and a specificity of 87% on all validation specimens. The prospectively collected specimens had a significantly higher 68% sensitivity

2018 International journal of cancer

199. Appropriate Handling, Processing and Analysis of Blood Samples Is Essential to Avoid Oxidation of Vitamin C to Dehydroascorbic Acid Full Text available with Trip Pro

in a selection of different clinical cohorts (diabetes, pneumonia, cancer, and critically ill). All clinical samples were collected into EDTA anticoagulant tubes and processed at 4 °C prior to storage at -80 °C for subsequent analysis by HPLC with electrochemical detection. We also investigated the effects of different handling and processing conditions on short-term and long-term ascorbate and DHA stability in vitro and in whole blood and plasma samples. These conditions included metal chelation (...) Appropriate Handling, Processing and Analysis of Blood Samples Is Essential to Avoid Oxidation of Vitamin C to Dehydroascorbic Acid Vitamin C (ascorbate) is the major water-soluble antioxidant in plasma and its oxidation to dehydroascorbic acid (DHA) has been proposed as a marker of oxidative stress in vivo. However, controversy exists in the literature around the amount of DHA detected in blood samples collected from various patient cohorts. In this study, we report on DHA concentrations

2018 Antioxidants

200. Capillary Blood Volume and Pain Perception Obtained in a Process of Puncturing With Different Safety Lancets

way and was performed by medical staff trained from lancing procedure described in the Protocol. Total blood collection time was recorded in the source documentation and then volume of the blood was measured using a calibrated ruler. Other Name: Cambridge Sensors lancet Outcome Measures Go to Primary Outcome Measures : Volume (microliters) of blood in the capillary tube [ Time Frame: The blood volume was noted after 20 seconds ] The amount of capillary blood volume collected after a single lancing (...) of the fingertip. Volume (microliters) of blood in the capillary tube [ Time Frame: The blood volume was noted until the end of bleeding or up to 2 minutes time ] The amount of capillary blood volume collected after a single lancing of the fingertip. Secondary Outcome Measures : Pain perception [ Time Frame: directly after lancing ] The intensity of pain perceived by the patient was assessed with the use of the numeric Visual Analog Scale (VAS). The Subject marked on the scale the number from 0 ("no pain

2018 Clinical Trials

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