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181. Quantitative High-Throughput Screening Using a Coincidence Reporter Biocircuit Full Text available with Trip Pro

Quantitative High-Throughput Screening Using a Coincidence Reporter Biocircuit Reporter-biased artifacts-i.e., compounds that interact directly with the reporter enzyme used in a high-throughput screening (HTS) assay and not the biological process or pharmacology being interrogated-are now widely recognized to reduce the efficiency and quality of HTS used for chemical probe and therapeutic development. Furthermore, narrow or single-concentration HTS perpetuates false negatives during primary

2017 Current Protocols in Neuroscience

182. Obstructive Sleep Apnea Screening Using a Piezo-Electric Sensor Full Text available with Trip Pro

Obstructive Sleep Apnea Screening Using a Piezo-Electric Sensor In this study, we propose a novel method for obstructive sleep apnea (OSA) detection using a piezo-electric sensor. OSA is a relatively common sleep disorder. However, more than 80% of OSA patients remain undiagnosed. We investigated the feasibility of OSA assessment using a single-channel physiological signal to simplify the OSA screening. We detected both snoring and heartbeat information by using a piezo-electric sensor

2017 Journal of Korean medical science

183. Analysis of DFS70 pattern and impact on ANA screening using a novel HEp-2 ELITE/DFS70 knockout substrate Full Text available with Trip Pro

Analysis of DFS70 pattern and impact on ANA screening using a novel HEp-2 ELITE/DFS70 knockout substrate Indirect immunofluorescence (IIF) using human epithelial cell (HEp-2) substrate is a widely used and the recommended method for screening of antinuclear antibodies (ANA). Dense fine speckled (DFS70) pattern on HEp-2 has been widely reported in various healthy and disease groups. Interpretation of DFS70 pattern can be challenging on a conventional HEp-2 substrate due to its similarity to some

2017 Auto-Immunity Highlights

184. CRISPR/Cas9 screening using unique molecular identifiers Full Text available with Trip Pro

CRISPR/Cas9 screening using unique molecular identifiers Loss-of-function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, Random Sequence Labels (RSLs) are incorporated into the guide library, which act as unique molecular identifiers (UMIs) to allow massively parallel lineage tracing and lineage dropout screening. RSLs greatly improve (...) the reproducibility of results by increasing both the precision and the accuracy of screens. They reduce the number of cells needed to reach a set statistical power, or allow a more robust screen using the same number of cells.© 2017 The Authors. Published under the terms of the CC BY 4.0 license.

2017 Molecular systems biology

185. High-throughput screens using photo-highlighting discover BMP signaling in mitochondrial lipid oxidation Full Text available with Trip Pro

High-throughput screens using photo-highlighting discover BMP signaling in mitochondrial lipid oxidation High-throughput screens at microscopic resolution can uncover molecular mechanisms of cellular dynamics, but remain technically challenging in live multicellular organisms. Here we present a genetic screening method using photo-highlighting for candidate selection on microscopes. We apply this method to stimulated Raman scattering (SRS) microscopy and systematically identify 57

2017 Nature communications

186. Telemedicine for diabetic retinopathy screening using an ultra-widefield fundus camera Full Text available with Trip Pro

Telemedicine for diabetic retinopathy screening using an ultra-widefield fundus camera Telemedicine reporting of diabetic retinopathy (DR) screening using ultra-widefield (UWF) fundus camera.Cross-sectional study of diabetic patients who visited the endocrinology department of a private multi-specialty hospital in United Arab Emirates between April 2015 and January 2017 who underwent UWF fundus imaging. Fundus pictures are then accessed at the Retina Clinic in the Department of Ophthalmology (...) %), and proliferative DR (PDR) in 13 eyes (7.88%). The secondary outcome measure of poor image acquisition was seen in one individual who had an image acquired in one eye that could not be graded due to bad picture quality.The present study has shown the effectiveness of DR screening using UWF fundus camera. It has shown the effectiveness of trained nursing personnel taking fundus images. This model can be replicated in any private multi-specialty hospital and reduce the burden of DR screening in the retina clinic

2017 Clinical ophthalmology (Auckland, N.Z.)

187. Adverse childhood experience and mental health screening using home visiting programs: a pilot study Full Text available with Trip Pro

Adverse childhood experience and mental health screening using home visiting programs: a pilot study Adverse childhood experiences (ACEs) are associated with adult high-risk behaviors and diseases. There is value in screening parents for ACEs given the repercussions parental ACEs may have on parenting behaviors and child development. The primary aim of this study was to assess the feasibility of parental ACE screening in the home setting. A secondary aim was to evaluate whether or not maternal

2017 Public health nursing (Boston, Mass.)

188. Frequency-dependent drug screening using optogenetic stimulation of human iPSC-derived cardiomyocytes Full Text available with Trip Pro

Frequency-dependent drug screening using optogenetic stimulation of human iPSC-derived cardiomyocytes Side effects on cardiac ion channels are one major reason for new drugs to fail during preclinical evaluation. Herein we propose a simple optogenetic screening tool measuring extracellular field potentials (FP) from paced cardiomyocytes to identify drug effects over the whole physiological heart range, which is essential given the rate-dependency of ion channel function and drug action. Human

2017 Scientific reports

189. Rapid detection of fentanyl, fentanyl analogues, and opioids for on-site or laboratory based drug seizure screening using thermal desorption DART-MS and ion mobility spectrometry Full Text available with Trip Pro

Rapid detection of fentanyl, fentanyl analogues, and opioids for on-site or laboratory based drug seizure screening using thermal desorption DART-MS and ion mobility spectrometry Fentanyl and fentanyl analogues represent a current and emerging threat in the United States as pure illicit narcotics and in mixtures with heroin. Because of their extreme potency, methods to safely and rapidly detect these compounds are of high interest. This work investigates the use of thermal desorption direct

2017 Forensic chemistry

190. Monkeypox Virus Host Factor Screen Using Haploid Cells Identifies Essential Role of GARP Complex in Extracellular Virus Formation Full Text available with Trip Pro

Monkeypox Virus Host Factor Screen Using Haploid Cells Identifies Essential Role of GARP Complex in Extracellular Virus Formation Monkeypox virus (MPXV) is a human pathogen that is a member of the Orthopoxvirus genus, which includes Vaccinia virus and Variola virus (the causative agent of smallpox). Human monkeypox is considered an emerging zoonotic infectious disease. To identify host factors required for MPXV infection, we performed a genome-wide insertional mutagenesis screen in human

2017 Journal of virology

191. Pediatric vision screening using the plusoptiX A12C photoscreener in Chinese preschool children aged 3 to 4 years Full Text available with Trip Pro

Pediatric vision screening using the plusoptiX A12C photoscreener in Chinese preschool children aged 3 to 4 years This study evaluated the performance of plusoptiX A12C in detecting amblyopia risk factors (ARFs) in Chinese children aged 3-to-4-year. PlusoptiX examination was successfully conducted among 1,766 subjects without cycloplegia to detect refractive error, asymmetry and media opacity. Cycloplegic retinoscopy (CR) was conducted on 357 children suspected of having vision abnormalities

2017 Scientific reports

192. Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7 Full Text available with Trip Pro

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7 Enterohemorrhagic Escherichia coli O157:H7 has been linked to both waterborne and foodborne illnesses, and remains a threat despite the food- and water-screening methods used currently. While conventional bacterial detection methods, such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assays (ELISA) can specifically detect pathogenic contaminants, they require extensive

2017 Journal of visualized experiments : JoVE

193. Simultaneous multi-gene mutation screening using SNPscan in patients from ethnic minorities with nonsyndromic hearing-impairment in Northwest China Full Text available with Trip Pro

Simultaneous multi-gene mutation screening using SNPscan in patients from ethnic minorities with nonsyndromic hearing-impairment in Northwest China The present study aimed to investigate the molecular etiology of nonsyndromic hearing impairment (HI) in hearing impaired populations of Hui, Tibetan, and Tu ethnicities in northwest China. A total of 283 unrelated subjects with HI who attended special education schools in northwest China were enrolled in the present study. Single-nucleotide

2017 Molecular medicine reports

194. Kinase inhibitor screening using artificial neural networks and engineered cardiac biowires Full Text available with Trip Pro

Kinase inhibitor screening using artificial neural networks and engineered cardiac biowires Kinase inhibitors are often used as cancer targeting agents for their ability to prevent the activation of cell growth and proliferation signals. Cardiotoxic effects have been identified for some marketed kinase inhibitors that were not detected during clinical trials. We hypothesize that more predictive cardiac functional assessments of kinase inhibitors on human myocardium can be established

2017 Scientific reports

195. Acceptability of cervical cancer screening using visual inspection among women attending a childhood immunization clinic in Uganda Full Text available with Trip Pro

Acceptability of cervical cancer screening using visual inspection among women attending a childhood immunization clinic in Uganda To evaluate the acceptability and performance of cervical cancer (CC) screening using visual inspection with acetic acid (VIA) integrated into a rural immunization clinic in Uganda.We conducted a cross-sectional pilot study in rural Uganda. We explored associations between women's characteristics and acceptance of VIA testing. We collected samples for Papanicolaou

2017 Papillomavirus Research

196. Evaluation of critical congenital heart defects screening using pulse oximetry in the neonatal intensive care unit Full Text available with Trip Pro

Evaluation of critical congenital heart defects screening using pulse oximetry in the neonatal intensive care unit To evaluate the implementation of early screening for critical congenital heart defects (CCHDs) in the neonatal intensive care unit (NICU) and potential exclusion of sub-populations from universal screening.Prospective evaluation of CCHD screening at multiple time intervals was conducted in 21 NICUs across five states (n=4556 infants).Of the 4120 infants with complete screens, 92

2017 Journal of Perinatology

197. False-Positive Newborn Screen Using the Beutler Spot Assay for Galactosemia in Glucose-6-Phosphate Dehydrogenase Deficiency Full Text available with Trip Pro

False-Positive Newborn Screen Using the Beutler Spot Assay for Galactosemia in Glucose-6-Phosphate Dehydrogenase Deficiency Classical galactosemia is detected through newborn screening by measuring galactose-1-phosphate uridylyltransferase (GALT) in the USA primarily via the Beutler spot assay. We report on an 18-month-old patient with glucose-6-phosphate dehydrogenase (G6PD) deficiency that was originally diagnosed with classical galactosemia. The patient presented with elevated liver function

2017 JIMD reports

198. Preliminary Results: Colorectal Cancer Screening Using Fecal Immunochemical Test (FIT) in a Thai Population Aged 45-74 Years: A Population-Based Randomized Controlled Trial Full Text available with Trip Pro

Preliminary Results: Colorectal Cancer Screening Using Fecal Immunochemical Test (FIT) in a Thai Population Aged 45-74 Years: A Population-Based Randomized Controlled Trial Objective: The aim of this paper is to provide some details and the results to date of a colorectal cancer screening trial using a fecal immunochemical test (FIT). Methods: A population-based randomized controlled trial began in May, 2016. All people aged 45 to 74 years living in Nam Phong District, Khon Kaen Province

2017 Asian Pacific journal of cancer prevention : APJCP Controlled trial quality: uncertain

199. Reasons for Declining Preconception Expanded Carrier Screening Using Genome Sequencing Full Text available with Trip Pro

Reasons for Declining Preconception Expanded Carrier Screening Using Genome Sequencing Genomic carrier screening can identify more disease-associated variants than existing carrier screening methodologies, but its utility from patients' perspective is not yet established. A randomized controlled trial for preconception genomic carrier screening provided an opportunity to understand patients' decisions about whether to accept or decline testing. We administered a survey to potential genomic

2017 Journal of Genetic Counseling Controlled trial quality: uncertain

200. Improving discrimination in antepartum depression screening using the Edinburgh Postnatal Depression Scale. (Abstract)

Improving discrimination in antepartum depression screening using the Edinburgh Postnatal Depression Scale. Universal screening of pregnant women for postpartum depression has recently been recommended; however, optimal application of depression screening tools in stratifying risk has not been defined. The current study examines new approaches to improve the ability of the Edinburgh Postnatal Depression Scale (EPDS) to stratify risk for postpartum depression, including alternate cut points, use

2017 Journal of Affective Disorders

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